Effects of hypoxia on human lymphocyte activation

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7021995

Meehan, R; Duncan, U; Neale, L; Ramsey, K; Taylor, G

Peripheral blood mononuclear cells were obtained from 7 subjects at sea level (SL), 7500 ft (Alt/sub 1/) and 25,000 ft (Alt/sub 2/) during a 7 week decompression chamber study. Circulating leukocyte subpopulations were identified by flow cytometry using fluoresceinated monoclonal antibodies. In vitro PWM and PHA stimulated /sup 35/S Methionine incorporation was determined at 24 hours, /sup 3/H Thymidine uptake at 72 hours, and NK cytotoxicity at 24 hours by /sup 51/Cr released from K562 cells. An increase in percentages of monocytes (9 +/- 1 SL vs. 19 +/- 3 Alt/sub 2/) was observed without changes in T helper, T suppressor or B cells. NK cytotoxicity was unimpaired in 6 of 7 subjects at Alt/sub 2/ despite reduced PHA but not PWM stimulated protein synthesis at 24 hours and reduced blastogenesis at 72 hours. This study indicates that hypoxia may impair host resistance in humans by inducing immunoregulatory abnormalities.

• Research Organization: Univ. of Texas Medical Branch, Galveston
• OSTI ID: 7021995
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALTITUDE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANOXIA
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
CELL FLOW SYSTEMS
CELL PROLIFERATION
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
METHIONINE
MONOCLONAL ANTIBODIES
MONOCYTES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHORBOL ESTERS
PRIMATES
RADIOISOTOPES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES