A rapid in vitro screening system for the identification and evaluation of anticancer drugs

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Cancer Investigation; (USA)

DOI:https://doi.org/10.3109/07357908909039855· OSTI ID:7018981

Kao, J W; Collins, J L ^[1]

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1. Washington Univ. School of Medicine, St. Louis, MO (USA)

We report the development of an in vitro screening system that can be used to identify new anticancer drugs that are specifically cytotoxic for dividing cells. The screening system takes advantage of the potential of many cell lines, including tumor cells, to stop dividing when they are plated at high cell density. The cytotoxic effects of anticancer drugs on dividing (i.e., cells plated at low cell density) and nondividing cells (i.e., cells plated at high cell density) is measured by the incorporation of 51Cr. This in vitro system was evaluated by measuring the cytotoxic effects of the anticancer drugs cisplatin, thiotepa, doxorubicin, methotrexate, and vinblastine on the cell lines B/C-N, ME-180, and MCF-7. In this in vitro system the concentrations of the anticancer drugs that produced significant cytotoxicity on only dividing cells are similar to the concentrations that are used clinically. The fact that this in vitro system is rapid, simple, applicable to many cell types, and able to predict effective concentrations of anticancer drugs should make it useful for the screening of new anticancer drugs and for the design of preclinical studies.

• OSTI ID: 7018981
• Journal Information: Cancer Investigation; (USA), Journal Name: Cancer Investigation; (USA) Vol. 7:4; ISSN CINVD; ISSN 0735-7907
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTINEOPLASTIC DRUGS
AZINES
BETA DECAY RADIOISOTOPES
CHROMIUM 51
CHROMIUM ISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH
HETEROCYCLIC COMPOUNDS
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MAN
MICE
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RODENTS
SCREENING
THYMIDINE
TUMOR CELLS
VERTEBRATES