Dose critical in-vivo detection of anti-cancer drug levels in blood

Patent · Tue Jan 01 04:00:00 EST 1991

OSTI ID:867744

Miller, Holly H ^[1]

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1. Bethel Island, CA

A method and apparatus are disclosed for the in vivo and in vitro detection and measurement of dose critical levels of DNA-binding anti-cancer drug levels in biological fluids. The apparatus comprises a laser based fiber optic sensor (optrode) which utilizes the secondary interactions between the drug and an intercalating fluorochrome bound to a probe DNA, which in turn is attached to the fiber tip at one end thereof. The other end of the optical fiber is attached to an illumination source, detector and recorder. The fluorescence intensity is measured as a function of the drug concentration and its binding constant to the probe DNA. Anticancer drugs which lend themselves to analysis by the use of the method and the optrode of the present invention include doxorubicin, daunorubicin, carminomycin, aclacinomycin, chlorambucil, cyclophosphamide, methotrexate, 5-uracil, arabinosyl cytosine, mitomycin, cis-platinum 11 diamine dichloride procarbazine, vinblastine vincristine and the like. The present method and device are suitable for the continuous monitoring of the levels of these and other anticancer drugs in biological fluids such as blood, serum, urine and the like. The optrode of the instant invention also enables the measurement of the levels of these drugs from a remote location and from multiple samples.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• DOE Contract Number: W-7405-ENG-48
• Assignee: Regents of University of California (Oakland, CA)
• Patent Number(s): US 5001051
• OSTI ID: 867744
• Country of Publication: United States
• Language: English

Related Subjects

/435/356/385/436/
11
5-uracil
aclacinomycin
analysis
anti-cancer
anti-cancer drug
anticancer
apparatus
apparatus comprise
apparatus comprises
arabinosyl
attached
based
binding
biological
biological fluid
biological fluids
blood
bound
carminomycin
chlorambucil
cis-platinum
comprises
concentration
constant
continuous
continuous monitoring
critical
critical levels
cyclophosphamide
cytosine
daunorubicin
detection
detector
device
diamine
dichloride
disclosed
dna
dna-binding
dose
dose critical
doxorubicin
drug
drug levels
drugs
enables
fiber
fiber optic
fluids
fluorescence
fluorescence intensity
fluorochrome
function
illumination
illumination source
in-vivo
instant
intensity
interactions
intercalating
laser
laser based
lend
levels
location
measured
measurement
method
methotrexate
mitomycin
monitoring
multiple
multiple sample
multiple samples
optic
optic sensor
optical
optical fiber
optrode
probe
procarbazine
recorder
remote
remote location
samples
secondary
sensor
serum
source
suitable
tip
urine
utilizes
vinblastine
vincristine
vitro
vivo