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Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants: Mer genes and Hg (2+) resistance

Technical Report ·
OSTI ID:7018805
Nucleic acids extracted from microbial biomass were hybridized with probes representing four mer operons, to detect genes encoding adaptation to Hg{sup 2+}. An enrichment in sequences similar to the mer genes of transposon 501 occurred during adaptation in a freshwater community. In an estuarine community, all four mer genes were only slightly enriched, suggesting that additional, yet uncharacterized, mer genes encodeded adaptation to HG{sup 2+}.
Research Organization:
Environmental Protection Agency, Gulf Breeze, FL (USA). Environmental Research Lab.
OSTI ID:
7018805
Report Number(s):
PB-90-129529/XAB; EPA--600/J-89/167
Country of Publication:
United States
Language:
English

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