Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants: Mer genes and Hg2+ resistance
Journal Article
·
· Applied and Environmental Microbiology; (USA)
OSTI ID:5668295
- Environmental Protection Agency, Sabine Island, Gulf Breeze, FL (USA)
Nucleic acids extracted from microbial biomass without prior culturing were hybridized with probes representing four mer operons to detect genes encoding adaptation to Hg2+ in whole-community genomes. A 29-fold enrichment in sequences similar to the mer genes of transposon Tn501 occurred during adaptation in a freshwater community. In an estuarine community, all four mer genes were only slightly enriched (by three- to fivefold), suggesting that additional, yet uncharacterized, mer genes encoded adaptation to Hg2+.
- OSTI ID:
- 5668295
- Journal Information:
- Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 55:6; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL ADAPTATION
DNA HYBRIDIZATION
ELEMENTS
FRESH WATER
GENE OPERONS
GENES
HYBRIDIZATION
HYDROGEN COMPOUNDS
MERCURY
METALS
MICROORGANISMS
OXYGEN COMPOUNDS
POLLUTION
TOXICITY
WATER
WATER POLLUTION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL ADAPTATION
DNA HYBRIDIZATION
ELEMENTS
FRESH WATER
GENE OPERONS
GENES
HYBRIDIZATION
HYDROGEN COMPOUNDS
MERCURY
METALS
MICROORGANISMS
OXYGEN COMPOUNDS
POLLUTION
TOXICITY
WATER
WATER POLLUTION