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Comparative analysis of polychlorinated dibenzo-'p'-dioxin and dibenzofuran congeners in Great Lakes fish extracts by gas chromatography-mass spectrometry and in-vitro enzyme induction activities

Technical Report ·
OSTI ID:7018193
; ; ; ;
The high-resolution gas chromatographic-mass spectrometric analysis of 25 Great Lakes fish extracts confirmed the identities of several 2,3,7,8-tetrasubstituted polychlorinated dibenzofurans (PCDFs) and dibenzo-p-dioxins (PCDDs). The dominant congener in extracts from Lake Michigan, Lake Erie, Lake St. Clair, Lake Huron, and Lake Superior fish was 2,3,7,8-tetrachlorodibenzofuran (TCDF). The fish extracts from Lake Ontario showed significant levels of 2,3,7,8-tetrachlorobenzo-p-dioxin (TCDD) besides containing considerable amounts of PCDFs. The 2,3,7,8-TCDD equivalents in these extracts were determined by their activities as inducers of aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O-deethylase (EROD) in rat hepatoma H-4-II E cells in culture. For most of these samples, there was less than a 2-fold difference in the bioassay-estimated 2,3,7,8-TCDD equivalents and the total PCDDs plus PCDFs as determined by GC-MS. The bioassay-derived values were significantly (>2-fold) higher for the Lake Erie and Lake Ontario fish extracts. This difference may be due to several factors including synergistic interactive effects of the congeners in the bioassay induction response or the presence of bioassay-active components that are not detected by GC-MS analysis.
Research Organization:
Environmental Protection Agency, Duluth, MN (USA). Environmental Research Lab.
OSTI ID:
7018193
Report Number(s):
PB-90-127978/XAB
Country of Publication:
United States
Language:
English

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Related Subjects

54 ENVIRONMENTAL SCIENCES
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BIOASSAY
BIOLOGICAL MATERIALS
CHROMATOGRAPHY
DIOXIN
ENZYME INDUCTION
ENZYMES
FISHES
FURANS
GAS CHROMATOGRAPHY
GENE REGULATION
GREAT LAKES
HETEROCYCLIC COMPOUNDS
HYDROXYLASES
LAKES
MASS SPECTROSCOPY
MATERIALS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
POLLUTION
SEPARATION PROCESSES
SPECTROSCOPY
SURFACE WATERS
TISSUE EXTRACTS
TOXIC MATERIALS
TOXICITY
VERTEBRATES
WATER POLLUTION