Relative potency of polychlorinated dibenzo-p-dioxins and dibenzofurans for inducing mixed-function oxygenase activity in rainbow trout
- Univ. of Waterloo, Ontario (Canada). Dept. of Biology
- National Water Research Institute, Burlington, Ontario (Canada)
- Great Lakes Lab. for Fisheries and Aquatic Sciences, Burlington, Ontario (Canada)
Toxic equivalent factors (TEFs) were generated for various polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) to compare the relative potencies as inducers of liver mixed-function oxygenase (MFO) activity in rainbow trout (Oncorhynchus mykiss). Trout, dosed orally with one of five PCDD congeners, either [{sup 3}H]-2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 1,2,3,7,8-pentachlorodibenzo-p-dioxin (PnCDD), 1,2,3,6,7,8-hexachlorodibenzo-p-dioxin (1,2,3,6,7,8-HxCDD), [{sup 14}C]-1,2,3,4,7,8-hexachlorodibenzo-p-dioxin (1,2,3,4,7,8-HxCDD), or [{sup 14}C]-1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin (HpCDD) or one of four PCDF congeners, either 2,3,7,8-tetrachlorodibenzofuran (TCDF), 1,2,3,7,8-pentachlorodibenzofuran (1,2,3,7,8-PnCDF), 2,3,4,7,8-pentachlorodibenzofuran (2,3,4,7,8-PnCDF), or 1,2,3,4,7,8--hexachlorodibenzofuran (HxCDF), showed increases in liver ethoxyresorufin O-deethylase (EROD) activity up to 250-fold. Liver concentrations of congeners were better predictors of EROD activity than oral dose. TEFs, ratios of the potency of each congener to that of TCDD, were calculated comparing ``threshold`` PCDD concentrations that induced EROD significantly above control levels. In cases of nonparallel dose-response curves between congeners, these comparisons are more relevant to environmental situations as PCDDs are compared at concentrations closer to those in the environment, instead of at higher pharmaceutical levels. Threshold-based TEFs were: PnCDD, 1.8; 1,2,3,6,7,8-HxCDD and 1,2,3,4,7,8-HxCDD, 0.4; HpCDD, 0.05; 2,3,4,7,8-PnCDF, 2; TCDF, 0.5; 1,2,3,7,8-PnCDF and HxCDF, 0.4. The ranking of potencies of the PCDFs was the same in fish as in mammalian tests, with the exception of PnCDD and 2,3,4,7,8-PnCDF, which were more potent to fish than was TCDD.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 64553
- Journal Information:
- Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 6 Vol. 14; ISSN ETOCDK; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
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