Characterization of a high affinity interleukin-1 (IL-1) specific binding site in a human synovial sarcoma (Hs431) cell line
- Janssen Research Foundation, Spring House, PA (USA)
A subpopulation of cells was derived from the Hs431 connective tissue sarcoma cell line which possessed high affinity (estimated Kd = 0.38-0.55 nM) binding sites for human recombinant ({sup 125}I)-IL-1 alpha. Binding at 4{degrees}C was slow approaching equilibrium by 4 hrs. Dissociation of ({sup 125}I)-IL-1 alpha was also slow and unaffected by high concentrations of cold ligand. The binding site also underwent ligand-induced internalization at 37{degrees}C. An Mr = 83,000 protein was identified in affinity crosslinking studies. Despite these similarities to previously reported IL-1 receptors, Hs431 cells did not exhibit biological responses to IL-1 which have been observed in other cell lines. IL-1 did not induce PGE2 or collagenase synthesis. IL-1 also failed to induce ornithine decarboxylase activity (ODC) or stimulate ({sup 3}H)-thymidine incorporation. In contrast, the Hs431 cells did contain a functional epidermal growth factor (EGF) receptor as determined from binding studies, protein kinase activity, induction of ODC, and stimulation of ({sup 3}H)-thymidine incorporation. Thus, the refractoriness of Hs431 cells to IL-1 was fairly specific and did not result from a generalized defect associated with cell transformation.
- OSTI ID:
- 7016414
- Journal Information:
- Lymphokine Research; (USA), Vol. 9:2; ISSN 0277-6766
- Country of Publication:
- United States
- Language:
- English
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550201* - Biochemistry- Tracer Techniques