Fluorescence reagents for high-sensitivity chromatographic measurements of primary amines

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00168a025· OSTI ID:7014036

Beale, S C; Savage, J C; Wiesler, D; Wietstock, S M; Novotny, M

Model fluorogenic reagents have been synthesized and evaluated toward the goal of ultrahigh-sensitivity chromatographic analysis of primary amines with laser-based detection. Chemical reactivity and spectral criteria were utilized to evaluate 12 potential reagents forming highly fluorescent isoindoles. 3-Benzoyl-2-quinolinecarboxaldehyde has been chosen at this time as the most promising reagent for the precolumn derivatization of model amino acids, followed by a chromatographic analysis of the formed isoindoles, and their detection with a helium-cadmium laser-based detector at the femtogram level.

• Research Organization: Indiana Univ., Bloomington (USA)
• OSTI ID: 7014036
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:17; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
ALDEHYDES
AMINES
AROMATICS
AZAARENES
AZINES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
EMISSION SPECTROSCOPY
EXPERIMENTAL DATA
FLUORESCENCE
FLUORESCENCE SPECTROSCOPY
HETEROCYCLIC COMPOUNDS
INFORMATION
LASERS
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
QUANTITATIVE CHEMICAL ANALYSIS
QUINOLINES
SEPARATION PROCESSES
SPECTROSCOPY