Ultrastructural evidence for differentiation in a human glioblastoma cell line treated with inhibitors of eicosanoid metabolism

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Neurosurgery; (United States)

DOI:https://doi.org/10.1097/00006123-199010000-00003· OSTI ID:7013851

Wilson, D E; Anderson, K M ^[1]; Seed, T M ^[2]

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1. Rush Presbyterian St. Luke's Medical Center, Chicago, IL (United States)
2. Argonne National Lab., IL (United States)

Human glioblastoma cells incubated in the presence of inhibitors of eicosanoid biosynthesis show decreased cellular proliferation without cytotoxicity. The authors studied the ultrastructural morphology of a human glioblastoma cell line cultured with nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor, or 5,8,11,14-eicosatetraynoic acid, a cyclooxygenase and lipoxygenase inhibitor. When glioblastoma cells were treated for 3 days with antiproliferative concentrations of either agent, they shared many morphological characteristics, including evidence for increased astrocytic differentiation with only limited signs of toxicity. The inhibited glioma cells demonstrated an increase in the number and length of astrocytic processes containing greater numbers of glial filaments, and the NDGA-treated cells also demonstrated extensive lateral pseudopod formation along the processes. The glioblastoma cell shape also become more elongated, losing the usual nuclear lobularity and nuclear inclusions, especially in NDGA-treated cells. Many cytoplasmic organelles packed the cytosol of the inhibited glioma cells, including prominent Golgi apparatus, dilated smooth endoplasmic reticulum evolving into dilated vesicles, cytoplasmic vacuoles, and numerous concentric laminations. There was limited evidence for toxicity, however, as the mitochondria were more pleomorphic with some mitochondrial distension and disruption of the cristae along with an increase in cytoplasmic vacuolization. The authors conclude that the inhibitors of eicosanoid biosynthesis. NDGA and 5,8,11,14-eicosatetraynoic acid, not only suppress glioblastoma cell proliferation, but also include increased astrocytic differentiation.

• OSTI ID: 7013851
• Journal Information: Neurosurgery; (United States), Journal Name: Neurosurgery; (United States) Vol. 27:4; ISSN 0148-396X; ISSN NRSRD
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CELL DIFFERENTIATION
EICOSANOIC ACID
ENZYME INHIBITORS
INHIBITION
KINETICS
MAMMALS
MAN
METABOLISM
MONOCARBOXYLIC ACIDS
MORPHOLOGY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
REACTION KINETICS
TUMOR CELLS
VERTEBRATES