Pertussis toxin-catalyzed ADP-ribosylation of a G protein in mouse oocytes, eggs, and preimplantation embryos: Developmental changes and possible functional roles

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Developmental Biology; (USA)

DOI:https://doi.org/10.1016/0012-1606(90)90294-S· OSTI ID:7011533

Jones, J; Schultz, R M ^[1]

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1. Univ. of Pennsylvania, Philadelphia (USA)

G proteins, which in many somatic cells serve as mediators of signal transduction, were identified in preimplantation mouse embryos by their capacity to undergo pertussis toxin-catalyzed ADP-ribosylation. Two pertussis toxin (PT) substrates with Mr = 38,000 and 39,000 (alpha 38 and alpha 39) are present in approximately equal amounts. Relative to the amount in freshly isolated germinal vesicle (GV)-intact oocytes, the amount of PT-catalyzed ADP-ribosylation of alpha 38-39 falls during oocyte maturation, rises between the one- and two-cell stages, falls by the eight-cell and morula stages, and increases again by the blastocyst stage. The decrease in PT-catalyzed ADP-ribosylation of alpha 38-39 that occurs during oocyte maturation, however, does not require germinal vesicle breakdown (GVBD), since inhibiting GVBD with 3-isobutyl-1-methyl xanthine (IBMX) does not prevent the decrease in the extent of PT-catalyzed ADP-ribosylation. A biologically active phorbol diester (12-O-tetradecanoyl phorbol 13-acetate), but not an inactive one (4 alpha-phorbol 12,13-didecanoate, 4 alpha-PDD), totally inhibits the increase in PT-catalyzed ADP-ribosylation of alpha 38-39 that occurs between the one- and two-cell stage; TPA inhibits cleavage, but not transcriptional activation, which occurs in the two-cell embryo. In contrast, cytochalasin D, genistein, or aphidicolin, each of which inhibits cleavage of one-cell embryos, or alpha-amanitin or H8, each of which inhibits transcriptional activation but not cleavage of one-cell embryos, have little or inhibitory effects on the increase in PT-catalyzed ADP-ribosylation of alpha 38-39. Results of immunoblotting experiments using an antibody that is highly specific for alpha il-3 reveal the presence of a cross-reactive species of Mr = 38,000 (alpha 38) in the GV-intact oocyte, metaphase II-arrested egg, and one-, two-cell embryos.

• OSTI ID: 7011533
• Journal Information: Developmental Biology; (USA), Journal Name: Developmental Biology; (USA) Vol. 139:2; ISSN 0012-1606; ISSN DEBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
AMINO ACIDS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CARCINOGENS
CELL CONSTITUENTS
CELL DIFFERENTIATION
CELL MEMBRANES
CLEAVAGE
COENZYMES
CRYSTAL STRUCTURE
DRUGS
ELECTROPHORESIS
ESTERS
GERM CELLS
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
MAMMALS
MATERIALS
MEMBRANES
METABOLISM
METHIONINE
MICE
MICROSTRUCTURE
MOLECULAR WEIGHT
NAD
NUCLEOTIDES
ONTOGENESIS
OOCYTES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHORBOL ESTERS
PROTEINS
RODENTS
SULFUR ISOTOPES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRANSCRIPTION
VERTEBRATES