Studies on the metabolic fate of (/sup 14/C)2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the mouse

Journal Article · Fri Mar 30 04:00:00 EST 1984 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(84)90054-1· OSTI ID:7007970

Koshakji, R P; Harbison, R D; Bush, M T

After a single po dose (135 micrograms/kg; 62 microCi/kg) of /sup 14/C-labeled 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in male ICR/Ha Swiss mice, 67 to 76% of the administered dose was eliminated via the feces and 1 to 2% in the urine during the first 24 hr following treatment. It seems likely that most of this material was simply not absorbed. Much of the remaining chemical was then excreted slowly in the urine (2%) and feces (7%) during the next 10 days, partly as the unchanged compound and partly as metabolites. One of the metabolites (Fraction II) appears to be a single polar, acidic metabolite characterized in urine (0.4 +/- 0.1%) and feces (2.2 +/- 0.2%), and is also likely excreted as a glucuronide conjugate. The rest of the radioactivity was in the form of unchanged TCDD in the animal body (17 +/- 2%). Steady rates of decline in the concentrations of the /sup 14/C as well as of the unchanged TCDD were reached in the feces and urine after the fifth day following the administration of the chemical. Based on this steady rate, the half-life of the radioactivity in the body was approximately 20 days. Urine, feces, and whole body were analyzed by solvent extraction, /sup 14/C counting, thin-layer chromatography, and countercurrent distribution.

• Research Organization: Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee
• OSTI ID: 7007970
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 73:1; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
ANIMALS
BIOLOGICAL HALF-LIFE
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
DIOXANE
HETEROCYCLIC COMPOUNDS
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MICE
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RODENTS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
UPTAKE
VERTEBRATES