Contact hypersensitivity response to isophorone diisocyanate in mice

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Drug and Chemical Toxicology; (USA)

DOI:https://doi.org/10.3109/01480548908999159· OSTI ID:7004377

Stern, M L; Brown, T A; Brown, R D; Munson, A E ^[1]

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1. Virginial Commonwealth Univ., Richmond (USA)

Isophorone diisocyanate was evaluated for its potential as a sensitizing agent for allergic contact hypersensitivity in mice. Female B6C3F1 mice were sensitized with 0.1, 0.3, and 1.0% isophorone diisocyanate and challenged with 3.0% isophorone diisocyanate. Doses of isophorone diisocyanate were selected from assays for primary irritancy. Mice received 20 microliters by direct dermal application, for 5 days, to sites prepared by shaving, dermabrading and, in some mice, with intra dermal injection of complete Freund's adjuvant. The rest period was 7 days. Measurement of the contact hypersensitivity response in mice was by radioisotopic assay two days after challenge and mouse ear swelling one and two days after challenge. Mice demonstrated statistically significant dose-dependent contact hypersensitivity responses to isophorone diisocyanate with or without adjuvant pretreatment.

• OSTI ID: 7004377
• Journal Information: Drug and Chemical Toxicology; (USA), Journal Name: Drug and Chemical Toxicology; (USA) Vol. 12:3-4; ISSN DCTOD; ISSN 0148-0545
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
ALLERGY
ANIMALS
DERMATITIS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
IODINE ISOTOPES
ISOCYANATES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICE
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
RADIOASSAY
RODENTS
SKIN DISEASES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES