Mechanisms of contact photosensitivity in mice: II. Langerhans cells are required for successful induction of contact photosensitivity to TCSA

Journal Article · Sat May 01 00:00:00 EDT 1982 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12507465· OSTI ID:6929916

Miyachi, Y; Takigawa, M

The role of Langerhans cells in the induction of contact photosensitivity (CPS) to tetrachlorosalicylanilide (TCSA) was investigated in mice. CPS was induced by 2 daily paintings of 50 microliters of 1% TCSA in acetone plus black light irradiations for 2.5 hr. Both ears were challenged with 20 microliters of 0.1% TCSA in ethanol plus black light irradiation for 2.5 hr on day 5, and ear thickness was read at 24 hr. The density of LCs detected as ATPase-positive cells dramatically decreased 2 days after exposure to UVB. CPS was not induced by painting the photoallergen to the skin which had been pre-irradiated with UVB. The ear swelling response returned to the normal level when the mice were sensitized 12 days after UVB exposure in accordance with the complete regeneration of ATPase-positive cells. Dose of UVB in the present study did not affect the development of CPS through systemic mechanism. These demonstrations indicate that LCs play an important role in the induction of CPS.

• Research Organization: Department of Dermatology, Faculty of Medicine, Kyoto University, Japan
• OSTI ID: 6929916
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 78:5; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ANIMAL CELLS
ANIMALS
ATP-ASE
AUDITORY ORGANS
BODY
DERMATITIS
DISEASES
DOSES
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROLASES
MAMMALS
MEASURING METHODS
MICE
ORGANS
PHOSPHOHYDROLASES
RADIATION DOSES
RADIATIONS
RODENTS
SENSE ORGANS
SENSITIVITY
SKIN
SKIN DISEASES
ULTRAVIOLET RADIATION
VERTEBRATES