Accumulation of calcium and loss of potassium in the hippocampus following transient cerebral ischemia: a proton microprobe study

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1988.93· OSTI ID:7002659

Martins, E; Inamura, K; Themner, K; Malmqvist, K G; Siesjoe, B K

This study explored (a) whether postischemic accumulation of calcium in hippocampal neurons precedes or occurs pari passu with light microscopical signs of delayed neuronal necrosis, and (b) whether calcium initially accumulates in dendritic domains, presumed to have a high density of agonist-operated calcium channels. Transient ischemia of 10-min duration was induced in rats, and the animals were studied after 1, 2, 3, and 4 days of recovery. We measured total calcium and potassium contents in the stratum oriens, pyramidale, radiatum, and moleculare of the CA1 and CA3 sectors, using particle induced x-ray emission (PIXE) in the proton microprobe mode. The results showed significant accumulation of calcium and loss of potassium after 3 and 4 days of recovery in the CA1 sector, which developed neuronal necrosis, but not in the CA3 sector, which showed only occasional damage. In a few animals, calcium accumulation (and loss of potassium) was observed with no or only mild visible damage, but in the majority of animals the accumulation of calcium correlated to signs of neuronal necrosis. Since calcium accumulation was similar in all strata examined, the results failed to reveal preferential accumulation in dendritic or somal regions. Based on our results and those of Dux et al., we emphasize the possibility that delayed neuronal death is, at least in part, caused by increased calcium cycling of plasma membranes and gradual calcium overload of mitochondria.

• Research Organization: Univ. of Lund (Sweden)
• OSTI ID: 7002659
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Journal Name: J. Cereb. Blood Flow Metab.; (United States) Vol. 8:4; ISSN JCBMD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
ALKALINE EARTH METALS
ANIMAL CELLS
BARYONS
BIOLOGICAL ACCUMULATION
BODY
BRAIN
CALCIUM
CARDIOVASCULAR DISEASES
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHEMICAL ANALYSIS
DISEASES
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
HADRONS
HIPPOCAMPUS
ISCHEMIA
LOSSES
MEMBRANES
METALS
MITOCHONDRIA
NECROSIS
NERVE CELLS
NERVOUS SYSTEM
NONDESTRUCTIVE ANALYSIS
NUCLEONS
ORGANOIDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PIXE ANALYSIS
POTASSIUM
PROTONS
SOMATIC CELLS
VASCULAR DISEASES
X-RAY EMISSION ANALYSIS