Synthesis of a biological active tumor growth factor from the predicted DNA sequence of Shope fibroma virus

Journal Article · Tue Jul 26 04:00:00 EDT 1988 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00415a037· OSTI ID:7002629

Lin, Y; Caporaso, G; Chang, P; Ke, X; Tam, J P

A 55-residue peptide comprising the carboxyl portion (residues 26-80) of the Shope fibroma virus growth factor (SFGF), a predicted 80-residue DNA virus gene product that encoded a homologous sequence with the epidermal growth factor transforming growth factor ..cap alpha.. family, was synthesized by a stepwise solid-phase method. The synthetic SFGF (26-80) purified to homogeneity by reverse-phase HPLC was characterized by fission ionization mass spectrometry and amino acid analysis. The disulfide pairings were established by enzymatic digestion and mass spectrometry and were found to be similar to those of EGF and TGF..cap alpha... Synthetic SFGF (26-80) was found to share about 10% of the activities as EGF in the radioreceptor binding to A431 cells, stimulation of (/sup 3/H)thymidine uptake in NRK cells, and induction of colony formation in soft-agar assay. The results therefore confirmed that SFGF contained the putative biological activities of the EGF-TGF..cap alpha.. family and that production of SFGF by Shope fibroma virus infected cells may account for the proliferative diseases associated with this particular virus.

• Research Organization: Rockefeller Univ., New York, NY (USA)
• OSTI ID: 7002629
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:15; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
AZINES
BETA DECAY RADIOISOTOPES
BIOSYNTHESIS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ELECTRON CAPTURE RADIOISOTOPES
FIBROBLASTS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
MEMBRANE PROTEINS
MICROORGANISMS
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PEPTIDES
PROTEINS
PURIFICATION
PYRIMIDINES
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
RIBOSIDES
SEPARATION PROCESSES
SOMATIC CELLS
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VIRUSES