Synthesis of a biological active tumor growth factor from the predicted DNA sequence of Shope fibroma virus
A 55-residue peptide comprising the carboxyl portion (residues 26-80) of the Shope fibroma virus growth factor (SFGF), a predicted 80-residue DNA virus gene product that encoded a homologous sequence with the epidermal growth factor transforming growth factor ..cap alpha.. family, was synthesized by a stepwise solid-phase method. The synthetic SFGF (26-80) purified to homogeneity by reverse-phase HPLC was characterized by fission ionization mass spectrometry and amino acid analysis. The disulfide pairings were established by enzymatic digestion and mass spectrometry and were found to be similar to those of EGF and TGF..cap alpha... Synthetic SFGF (26-80) was found to share about 10% of the activities as EGF in the radioreceptor binding to A431 cells, stimulation of (/sup 3/H)thymidine uptake in NRK cells, and induction of colony formation in soft-agar assay. The results therefore confirmed that SFGF contained the putative biological activities of the EGF-TGF..cap alpha.. family and that production of SFGF by Shope fibroma virus infected cells may account for the proliferative diseases associated with this particular virus.
- Research Organization:
- Rockefeller Univ., New York, NY (USA)
- OSTI ID:
- 7002629
- Journal Information:
- Biochemistry; (United States), Vol. 27:15
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GROWTH FACTORS
AMINO ACID SEQUENCE
BIOSYNTHESIS
RADIORECEPTOR ASSAY
ONCOGENIC VIRUSES
DNA SEQUENCING
TRITIUM COMPOUNDS
FIBROBLASTS
IODINE 125
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
PEPTIDES
PURIFICATION
RECEPTORS
THYMIDINE
ANIMAL CELLS
AZINES
BETA DECAY RADIOISOTOPES
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MICROORGANISMS
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
SEPARATION PROCESSES
SOMATIC CELLS
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRACER TECHNIQUES
VIRUSES
550201* - Biochemistry- Tracer Techniques