Studies on the structure and expression of immunoglobulins bearing TEPC15 idiotopes
Thesis/Dissertation
·
OSTI ID:7002379
The phosphorylcholine (PC)-binding myeloma protein TEPC15 bears antigenic determinants termed idiotopes (Id) that serve as markers for clones of B-cells act as targets for regulation of these cells. Using monoclonal anti-Id antibodies as probes in solid phase radioimmunoassays, TEPC15 was shown to have at least 7 distinct, overlapping Id whose locations ranged from the antigen-binding region to the C/sub H/1 domain. Differences between the primary sequences in 23 PC-binding myeloma and hybridoma immunoglobulins (Ig) of known heavy chain sequence and light chain family were correlated with their effect on individual ID expression. These regions were than localized on a computer display of the X-ray crystallographic structure of a PC-binding myeloma protein McPC603.
- Research Organization:
- Texas Univ., Galveston (USA)
- OSTI ID:
- 7002379
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Fri Feb 28 23:00:00 EST 1986
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANTIBODIES
ANTIGENS
CHOLINE
DRUGS
GLOBULINS
HYDROXY COMPOUNDS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIPOTROPIC FACTORS
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PROTEINS
QUATERNARY COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANTIBODIES
ANTIGENS
CHOLINE
DRUGS
GLOBULINS
HYDROXY COMPOUNDS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIPOTROPIC FACTORS
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PROTEINS
QUATERNARY COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
TRACER TECHNIQUES