Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes

Title: Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes

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Abstract

Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings

Inventors:: Smith, R.E.; Dolbeare, F.A.

Publication Date:: 1980-10-21

OSTI Identifier:: 7000909

Patent Number(s):: US 4229528; A

Application Number:: PPN: US 5-959555

Assignee:: PTO; EDB-94-125898

DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Resource Relation:: Patent File Date: 13 Nov 1978

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; PEPTIDE HYDROLASES; BIOASSAY; CHEMICAL ANALYSIS; FLUORESCENCE; SUBSTRATES; ENZYMES; HYDROLASES; LUMINESCENCE; ORGANIC COMPOUNDS; PROTEINS; 550200* - Biochemistry

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                    Smith, R.E., and Dolbeare, F.A. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes.  United States: N. p., 1980. 
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                    Smith, R.E., & Dolbeare, F.A. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes.  United States.

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                    Smith, R.E., and Dolbeare, F.A. Tue .  
        "Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes".  United States.

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@article{osti_7000909,

  title        = {Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes},

  author       = {Smith, R.E. and Dolbeare, F.A.},

  abstractNote = {Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1980},

  month        = {10}

}

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Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes

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Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.
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