Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes
Patent
·
OSTI ID:7000909
Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- PTO; EDB-94-125898
- Patent Number(s):
- US 4229528; A
- Application Number:
- PPN: US 5-959555
- OSTI ID:
- 7000909
- Resource Relation:
- Patent File Date: 13 Nov 1978
- Country of Publication:
- United States
- Language:
- English
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Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes
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·
OSTI ID:7000909