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Title: Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes

Abstract

Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings

Inventors:
;
Publication Date:
OSTI Identifier:
7000909
Patent Number(s):
US 4229528; A
Application Number:
PPN: US 5-959555
Assignee:
PTO; EDB-94-125898
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 13 Nov 1978
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDE HYDROLASES; BIOASSAY; CHEMICAL ANALYSIS; FLUORESCENCE; SUBSTRATES; ENZYMES; HYDROLASES; LUMINESCENCE; ORGANIC COMPOUNDS; PROTEINS; 550200* - Biochemistry

Citation Formats

Smith, R.E., and Dolbeare, F.A. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes. United States: N. p., 1980. Web.
Smith, R.E., & Dolbeare, F.A. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes. United States.
Smith, R.E., and Dolbeare, F.A. Tue . "Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes". United States.
@article{osti_7000909,
title = {Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes},
author = {Smith, R.E. and Dolbeare, F.A.},
abstractNote = {Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1980},
month = {10}
}