Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes
Abstract
Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings
- Inventors:
- Publication Date:
- OSTI Identifier:
- 7000909
- Patent Number(s):
- US 4229528; A
- Application Number:
- PPN: US 5-959555
- Assignee:
- PTO; EDB-94-125898
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 13 Nov 1978
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; PEPTIDE HYDROLASES; BIOASSAY; CHEMICAL ANALYSIS; FLUORESCENCE; SUBSTRATES; ENZYMES; HYDROLASES; LUMINESCENCE; ORGANIC COMPOUNDS; PROTEINS; 550200* - Biochemistry
Citation Formats
Smith, R E, and Dolbeare, F A. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes. United States: N. p., 1980.
Web.
Smith, R E, & Dolbeare, F A. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes. United States.
Smith, R E, and Dolbeare, F A. 1980.
"Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes". United States.
@article{osti_7000909,
title = {Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes},
author = {Smith, R E and Dolbeare, F A},
abstractNote = {Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings},
doi = {},
url = {https://www.osti.gov/biblio/7000909},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1980},
month = {10}
}
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