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Title: Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes

Abstract

Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings

Inventors:
;
Publication Date:
OSTI Identifier:
7000909
Patent Number(s):
US 4229528; A
Application Number:
PPN: US 5-959555
Assignee:
PTO; EDB-94-125898
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 13 Nov 1978
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDE HYDROLASES; BIOASSAY; CHEMICAL ANALYSIS; FLUORESCENCE; SUBSTRATES; ENZYMES; HYDROLASES; LUMINESCENCE; ORGANIC COMPOUNDS; PROTEINS; 550200* - Biochemistry

Citation Formats

Smith, R.E., and Dolbeare, F.A.. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes. United States: N. p., 1980. Web.
Smith, R.E., & Dolbeare, F.A.. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes. United States.
Smith, R.E., and Dolbeare, F.A.. 1980. "Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes". United States. doi:.
@article{osti_7000909,
title = {Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes},
author = {Smith, R.E. and Dolbeare, F.A.},
abstractNote = {Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1980,
month =
}