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Title: Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes

Abstract

Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 5-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.

Inventors:
 [1];  [2]
  1. (557 Escondido Cir., Livermore, CA 94550)
  2. (5178 Diane La., Livermore, CA 94550)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
863356
Patent Number(s):
US 4155916
Assignee:
Smith, Robert E. (557 Escondido Cir., Livermore, CA 94550);Dolbeare, Frank A. (5178 Diane La., Livermore, CA 94550) LLNL
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
fluorescence; method; enzyme; analysis; couples; aromatic; amines; aldehydes; proteinases; accomplished; conventional; amino; acid; containing; amine; substrates; 4-methoxy-2-naphthylamine; 4m2na; 2-naphthylamine; aminoisophthalic; dimethyl; ester; p-nitroaniline; 5-methoxy-1-aminofluorene; coumarin; derivatives; resulting; enzymatic; hydrolysis; substrate; 5-nitrosalicylaldehyde; 5-nsa; benzaldehyde; p-nitrobenzaldehyde; produce; schiff-base; complexes; water; insoluble; shift; blue; orange-red; visible; useful; assay; enzymes; acid containing; aromatic amines; amino acid; enzymatic hydrolysis; water insoluble; aromatic aldehydes; enzyme analysis; fluorescence method; coumarin derivatives; ethyl ester; methyl ester; aromatic amine; amine substrates; conventional amino; couples aromatic; containing aromatic; /999/435/

Citation Formats

Smith, Robert E., and Dolbeare, Frank A. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes. United States: N. p., 1979. Web.
Smith, Robert E., & Dolbeare, Frank A. Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes. United States.
Smith, Robert E., and Dolbeare, Frank A. 1979. "Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes". United States. doi:. https://www.osti.gov/servlets/purl/863356.
@article{osti_863356,
title = {Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes},
author = {Smith, Robert E. and Dolbeare, Frank A.},
abstractNote = {Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 5-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1979,
month = 1
}

Patent:

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