Human endothelial cells synthesize and express and Arg-Gly-Asp-directed adhesion receptor involved in attachment to fibrinogen and von Willebrand factor

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.18.6471· OSTI ID:6999927

Cheresh, D A

Human umbilical vein endothelial cells express a heterodimeric adhesion receptor complex consisting of noncovalently associated ..cap alpha.. and ..beta.. subunits that under reducing conditions have molecular masses of 135 kDa and 115 kDa, respectively, This complex can be isolated in pure form from an affinity matrix consisting of an Arg-Gly-Asp-containing heptapeptide and is specifically immunoprecipitated with monoclonal antibodies (mAbs) directed against the vitronectin receptor of human melanoma cells. These data suggest that this complex is one member of a large family of cell adhesion receptors. One of the mAbs, LM609, inhibits the attachment of human endothelial cells to fibrinogen, von Willebrand factor, and vitronectin yet has no effect on the attachment of these cells to fibronectin, collagen, or laminin. In addition, mAb LM609 inhibits attachment of endothelial cells to an immobilized synthetic peptide containing the Arg-Gly-Asp sequence. This adhesion receptor appears structurally similar to the IIb/IIIa glycoprotein complex expressed on platelets yet is antigenically distinct, since mAb LM609 fails to recognize IIb/IIIa glycoproteins. This receptor organizes in clusters on endothelial cells during their attachment to von Willebrand factor, vitronectin, or the Arg-Gly-Asp-containing heptapeptide. The data presented in this report suggest that Arg-Gly-Asp recognition may play a significant role in biological events associated with vascular proliferation.

• Research Organization: Scripps Clinic and Research Foundation, La Jolla, CA (USA)
• OSTI ID: 6999927
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:18; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMIDES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
ARGININE
ASPARAGINE
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BLOOD COAGULATION FACTORS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL PROLIFERATION
COAGULANTS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTROPHORESIS
ENDOTHELIUM
EVEN-ODD NUCLEI
FIBRINOGEN
FLUORESCENCE
GLOBULINS
GLUCOPROTEINS
GLYCINE
HEMATOLOGIC AGENTS
HEMOSTATICS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
MAMMALS
MAN
MELANOMAS
MEMBRANE PROTEINS
METHIONINE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
SACCHARIDES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TISSUES
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES