Detection of disease-specific restriction fragment length polymorphisms in pemphigus vulgaris linked to the DQwl and DQw3 alleles of the HLA-D region
Pemphigus vulgaris in Israeli Ashkenazi and non-Ashkenazi Jews and in Austrian non-Jewish patients is strongly associated with the DR4 and DRw6 alleles of the HLA-D region class II genes. Restriction fragment length polymorphism analysis was undertaken with DQ..beta.., DQ..cap alpha.., and DR..beta.. cDNA probes. Hybridization with the DQ..beta.. probe identifies Pvu II, BamHI, and EcoRV fragments that absolutely discriminate pemphigus vulgaris patients from healthy DR-, DQ-, and ethnic-matched controls. In contrast the DQ..cap alpha.. and DR..beta.. probes failed to identify disease-specific restriction fragment length polymorphism fragments. These studies indicate that DQw1 and DQw3 polymorphisms carried by pemphigus vulgaris patients may be directly involved in predisposition to the disease or may be tightly linked to the susceptibility gene itself. To our knowledge, this is the first example of an HLA restriction fragment length polymorphism that is highly associated with susceptibility to autoimmune disease.
- Research Organization:
- Hebrew Univ., Jerusalem (Israel)
- OSTI ID:
- 6999783
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:18
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
RECOMBINANT DNA
AUTORADIOGRAPHY
ELECTROPHORESIS
RFLPS
GENETICS
ATP
HEREDITARY DISEASES
HUMAN POPULATIONS
HYBRIDIZATION
IMMUNITY
PATIENTS
PHOSPHORUS 32
SKIN DISEASES
BETA DECAY RADIOISOTOPES
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DISEASES
DNA
ISOTOPES
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NUCLEI
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ODD-ODD NUCLEI
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PHOSPHORUS ISOTOPES
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