Molecular variation of human major histocompatibility complex DQw3. beta. -chains

Journal Article · Sun Nov 15 04:00:00 EST 1987 · J. Immunol.; (United States)

OSTI ID:5303384

So, A K.L.; Lindsay, J; Bodmer, J; Trowsdale, J

Histocompatibility leukocyte antigen DQ molecules exhibit polymorphism of both DQ..cap alpha..- and ..beta..-chains. Histocompatibility leukocyte antigen-DQw3 is associated with both DR4 and DR5 and can be further subdivided by reactivity with the monoclonal antibody TA10. To determine the molecular nature of the DQ polymorphic alleles associated with the DR4 haplotype, the authors have sequenced and analyzed DQ..cap alpha.. and ..beta.. cDNA clones obtained from a DR4, Dw4, DQw3 cell line which is TA10-positive, using electrophoresis and autoradiography. The DQ..cap alpha..-chain sequence was identical to previously published sequences from the DR4 haplotype, but the DQ..beta.. sequence differed from published DR4-DQ..beta.. sequences obtained from DQw3-positive TA10-negative cell lines by eight amino acids, six of which were located in the ..beta..1 domain. Thus, the TA10 serologic determinants reside on the DQ..beta..-chain. A TA10-specific oligonucleotide probe was constructed based on the DQ..beta.. sequence, and its specificity was confirmed in a panel of TA10-positive and TA10-negative cell lines. An additional band was observed in Southern blotting experiments which may indicate a donor sequence for gene conversion.

• Research Organization: Imperial Cancer Research Fund Labs., London, England
• OSTI ID: 5303384
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 139:10; ISSN JOIMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ANTIGENS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DNA-CLONING
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
HUMAN POPULATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
MATERIALS
METHIONINE
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POPULATIONS
PRIMATES
RADIOISOTOPES
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
TWO-DIMENSIONAL ELECTROPHORESIS
VERTEBRATES