Radiation inactivation method provides evidence that membrane-bound mitochondrial creatine kinase is an oligomer
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Lyophilized suspensions of rabbit heart mitochondria have been irradiated with varying doses of gamma rays. Mitochondrial creatine kinase activity was inactivated exponentially with a radiation inactivation size of 352 or 377 kDa depending upon the initial medium. These values are in good agreement with the molecular mass previously deduced from by permeation experiments: 357 kDa. This is the first direct evidence showing that the native form of mitochondrial creatine kinase is associated to the inner membrane as an oligomer, very likely an octamer.
- OSTI ID:
- 6999589
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 153:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Three-step preparation and purification of phosphorus-33-labeled creatine phosphate of high specific activity
Rate equation for creatine kinase predicts the in vivo reaction velocity: /sup 31/P NMR surface coil studies in brain, heart, and skeletal muscle of the living rat
Kinetics of the creatine kinase reaction in neonatal rabbit heart: An empirical analysis of the rate equation
Journal Article
·
Thu Mar 01 00:00:00 EST 1984
· Anal. Biochem.; (United States)
·
OSTI ID:6999589
Rate equation for creatine kinase predicts the in vivo reaction velocity: /sup 31/P NMR surface coil studies in brain, heart, and skeletal muscle of the living rat
Journal Article
·
Tue Sep 22 00:00:00 EDT 1987
· Biochemistry; (United States)
·
OSTI ID:6999589
Kinetics of the creatine kinase reaction in neonatal rabbit heart: An empirical analysis of the rate equation
Journal Article
·
Tue Mar 12 00:00:00 EST 1991
· Biochemistry; (United States)
·
OSTI ID:6999589
+1 more
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MITOCHONDRIA
CHEMICAL RADIATION EFFECTS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
CELL MEMBRANES
GAMMA RADIATION
HEART
INHIBITION
RABBITS
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
IONIZING RADIATIONS
MAMMALS
MEMBRANES
ORGANOIDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
TRANSFERASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
MITOCHONDRIA
CHEMICAL RADIATION EFFECTS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
CELL MEMBRANES
GAMMA RADIATION
HEART
INHIBITION
RABBITS
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
IONIZING RADIATIONS
MAMMALS
MEMBRANES
ORGANOIDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
TRANSFERASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture