Complete inhibition of creatine kinase in isolated perfused rat hearts
Transient exposure of an isolated isovolumic perfused rat heart to low concentrations (0.5 mM) of perfusate-born iodoacetamide resulted in complete inhibition of creatine kinase and partial inhibition of glyceraldehyde-3-phosphate dehydrogenase in the heart. At low levels of developed pressure, hearts maintained mechanical function, ATP, and creatine phosphate levels at control values. However, iodoacetamide-inhibited hearts were unable to maintain control values of end diastolic pressure or peak systolic pressure as work load increased. Global ischemia resulted in loss of all ATP without loss of creatine phosphate, indicating lack of active creatine kinase. These results indicate that isovolumic perfused rat hearts are able to maintain normal function and normal levels of high-energy phosphates without active creatine kinase at low levels of developed pressure. /sup 31/P-NMR of the heart was carried out.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 6352364
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 252:1
- Country of Publication:
- United States
- Language:
- English
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HEART
NUCLEAR MAGNETIC RESONANCE
PHOSPHOTRANSFERASES
BIOCHEMISTRY
INHIBITION
ACETAMIDE
BLOOD PRESSURE
CREATINE
HEMIACETAL DEHYDROGENASES
ISCHEMIA
NMR SPECTRA
ORGANIC IODINE COMPOUNDS
PERFUSED TISSUES
PHOSPHORUS 31
RATS
AMIDES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHEMISTRY
DISEASES
ENZYMES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RESONANCE
RODENTS
SPECTRA
STABLE ISOTOPES
TISSUES
TRANSFERASES
VASCULAR DISEASES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics