Direct assays of radiation induced DNA base lesions in mammalian cells: Technical progress report

Technical Report · Tue Dec 31 23:00:00 EST 1985

OSTI ID:6998424

Wheeler, K T

HPLC techniques were used to separate, detect, identify and quantitate radiation-induced damage products of adenine (A)-containing model compounds and eukaryotic DNA. A library of 7 pure synthetic or isolated major damage products has been established consisting of 8-hydroxyadenine, 4,6 diamino-5-formamidopyrimidine, 8-hydroxy-2-deoxyadenosine, 8 hydroxy-2-deoxadenosine monophosphate, the R and S isomers of 8,5'-cyclo-2-deoxyadenosine monophosphate and ..cap alpha..-deoxyadenosine. The structure and conformation may drastically alter the spectrum and quantity of lesions produced by ionizing radiation. This had been suggested by others on the basis of the results observed when the dinucleotides dApT and dTpA were irradiated in dilute solution. When poly (dA) and ds poly d(A-T) were irradiated under oxic conditions, the major damage product (8-OHdAdo) was the same, but the yield was different. When these compounds were irradiated under anoxic conditions, the cdAdo isomers were not the major damage products but rather ..cap alpha..-dAdo was produced in quantities 10 to 100 times greater than the cdAdo isomers. 2 refs., 1 fig., 2 tabs.

• Research Organization: Bowman Gray School of Medicine, Winston-Salem, NC (USA). Dept. of Radiology
• DOE Contract Number: AC02-84ER60196; FG05-86ER60464
• OSTI ID: 6998424
• Report Number(s): DOE/ER/60464-1; ON: DE87003919
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CHROMATOGRAPHY
DATA
DNA
EXPERIMENTAL DATA
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
NUCLEIC ACIDS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATION CHEMISTRY
RADIATION EFFECTS
SEPARATION PROCESSES