(Direct assays of radiation-induced DNA base lesions in mammalian cells. ) Final progress report, February 1, 1984-June 30, 1986

Technical Report · Wed Jan 01 04:00:00 EST 1986

OSTI ID:5218200

Wheeler, K T

Adenine (Ade), 2'-deoxyadenosine (dAdo) and 5'-deoxyadenosine monophosphate (5'-dAMP) were irradiated with 50 to 15,000 Gy under oxic and hypoxic conditions. HPLC procedures providing satisfactory separation of the adenine damage products formed during irradiation of DNA model compounds were found. Structures of some of the damage products were confirmed to include 8-OHAde, 4,6-diamino-5-formamidopyrimidine, and 8-OH-5'-dAMP. Two damage products of dAdo (8-OHdAdo and the major isomer of 8,5'-cdAdo), the formation of which depends on the presence or absence of oxygen, were determined quantitatively by HPLC. The limit for HPLC detection was estimated as 4 to 50 pmoles for these compounds. This corresponds to a detection limit of about 50 Gy in radiation dose units. These two products were also detected in mixtures of all four nucleosides irradiated with 50 Gy.

• Research Organization: Kansas Univ., Lawrence (USA)
• DOE Contract Number: AC02-84ER60196
• OSTI ID: 5218200
• Report Number(s): DOE/ER/60196-T1; ON: DE86015253
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOLOGY
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CHROMATOGRAPHY
DNA
DRUGS
HETEROCYCLIC COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
NMR SPECTRA
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOBIOLOGY
RIBOSIDES
SEPARATION PROCESSES
SPECTRA
SPECTROSCOPY