Transcriptional activation of the lipoprotein lipase and apolipoprotein E genes accompanies differentiation in some human macrophage-like cell lines

Journal Article · Tue Apr 19 04:00:00 EDT 1988 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00408a003· OSTI ID:6997417

Auwerx, J H; Deeb, S; Brunzell, J D; Peng, R; Chait, A

Stimulation of the macrophage-like cell line THP-1 with the phorbol ester phorbol 12-myristate 13-acetate (PMA) resulted in differentiation into cells with many features of macrophages. This differentiation was accompanied by transcriptional activation of the lipoprotein lipase (LPL) and apo E genes and accumulation of their protein products in the media. PMA-induced differentiation of the HEL and HL-60 cell lines was not accompanied by induction of the gene for LPL, whereas the apo E gene was induced slightly in HL-60 cells. By contrast, the gene for superoxide dismutase (SOD-1) was either unaffected (THP-1) or down regulated (HL-60 or HEL cells) by PMA treatment. Induction of LPL mRNA in THP-1 cells as dependent upon the concentration of phorbol ester added. A minimal concentration of 1.6 x 10/sup -8/ M PMA was necessary for macrophage differentiation, induction of LPL mRNA, and synthesis of the enzyme. LPL mRNA accumulates within 3 h after stimulation with PMA and attains a maximum concentration after 6 h, thereafter slowly decreasing over the next 3 days. In contrast, the steady-state level of apo E mRNA in the same THP-1 cultures increased gradually over a period of 48 h after induction. These studies thus demonstrate that THP-1 cells are of value as a model to study the quantitative and temporal expression of the LPL and apo E genes during macrophage differentiation.

• Research Organization: Univ. of Washington, Seattle (USA)
• OSTI ID: 6997417
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:8; ISSN BICHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
APOLIPOPROTEINS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOXYLESTERASES
CARCINOGENS
CELL DIFFERENTIATION
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
ESTERASES
ESTERS
GENES
HYDROLASES
ISOTOPES
LIGHT NUCLEI
LIPASE
LIPIDS
LIPOPROTEINS
MACROPHAGES
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
SOMATIC CELLS
TRANSCRIPTION