Molecular cloning of a gene selectively induced by gamma interferon from human macrophage cell line U937
Journal Article
·
· Mol. Cell. Biol.; (United States)
A cDNA clone encoding a gamma interferon (IFN-/gamma/)-inducible mRNA in human cells of the macrophage lineage was isolated and characterized. The corresponding gene, /gamma/.1, was selectively induced by IFN-/gamma/, responding a hundredfold better to IFN-/gamma/ than to IFN-/alpha/. The induction was rapid and transient, with maximal mRN accumulation at about 3 h and decline to the basal level after 48 h. Transcriptional activation could be detected as early as 5 min after IFN-/gamma/ stimulation and accounted entirely for the mRNA accumulation. The induction of /gamma/.1 by IFN-/gamma/ was cell-type restricted, being seen only in macrophages and endothelial cells. In addition, phorbol ester-induced differentiation of promyelocytic HL-60 cells and promonocytic THP-1 cells rendered the /gamma/.1 gene inducible by IFN-/gamma/. The 1.0-kilobsase /gamma/.1 cDNA sequence encoded a small predicted polypeptide of 38 amino acids and had a conserved sequence associated with rapidly turning over mRNAs. In vitro transmission of the /gamma/.1 transcript yielded a 4,000-dalton polypeptide.
- Research Organization:
- Albert Einstein Coll. of Medicine, Bronx, NY (USA); Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC 2 (GB)
- OSTI ID:
- 5826616
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 9:5; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARCINOGENS
CELL CULTURES
CELL DIFFERENTIATION
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
DNA-CLONING
ENZYME INDUCTION
ESTERS
GENE REGULATION
GENES
GROWTH FACTORS
HYBRIDIZATION
IN VITRO
INTERFERON
LYMPHOKINES
MACROPHAGES
MESSENGER-RNA
MITOGENS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
PHORBOL ESTERS
PROTEINS
RNA
RNA PROCESSING
SOMATIC CELLS
SYNTHESIS
TRANSCRIPTION
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARCINOGENS
CELL CULTURES
CELL DIFFERENTIATION
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
DNA-CLONING
ENZYME INDUCTION
ESTERS
GENE REGULATION
GENES
GROWTH FACTORS
HYBRIDIZATION
IN VITRO
INTERFERON
LYMPHOKINES
MACROPHAGES
MESSENGER-RNA
MITOGENS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
PHORBOL ESTERS
PROTEINS
RNA
RNA PROCESSING
SOMATIC CELLS
SYNTHESIS
TRANSCRIPTION