T-cell activation in pulmonary lymph nodes of mice exposed to ozone

Journal Article · Sun Dec 01 04:00:00 EST 1985 · Environ. Res.; (United States)

OSTI ID:6997358

Dziedzic, D; White, H J

Groups of Cd-1 female mice were exposed to ozone at 0.3, 0.5, and 0.7 ppm, 20 hr per day, 7 days per week for 1-28 days. The effect of ozone exposure on lymphoid cells was determined by studying mediastinal lymph nodes at various times of exposure. It was found that lymphocyte numbers underwent a dose-dependent, four-phased change:cellular depletion (Days 1-2), followed by rapid hyperplasia (Days 3-4), incremental cell number reduction (Days 5-7), and a subsequent subacute phase of elevated lymphocyte numbers (Days 8-28). Using tritiated thymidine it was determined that cells underwent a rapid burst of division by Day 3 of exposure and that mitosis subsequently declined to near baseline values by 2 weeks of exposure. Autoradiographic analysis of histologic sections revealed that the paracortical T-cell areas of the nodes were particularly involved. In addition to the increase in thymidine uptake, several morphologic changes were evident in affected cells. By comparison, the B cells from ozone-exposed animals were virtually unaffected with respect to cell division or morphological alterations. Prior treatment of ozone-exposed animals with a monoclonal antibody that is cytotoxic for T cells eliminated the hyperplastic response. Immunologic aspects of T-cell reactivity were studied. T-cell responsiveness to mitogenic stimulation with concanavalin A showed little alteration during the first days of exposure; however, by Day 14 an increase in reactivity was observed. This change indicated that functional lymphocyte stimulation occurred during ozone exposure.

• Research Organization: General Motors Research Labs., Warren, MI
• OSTI ID: 6997358
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 38:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIVISION
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
IMMUNE REACTIONS
INHALATION
INTAKE
LABELLED COMPOUNDS
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MITOSIS
MONOCLONAL ANTIBODIES
MORPHOLOGICAL CHANGES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OZONE
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMIDINE
TOXICITY
TRITIUM COMPOUNDS
VERTEBRATES