Possible site of calcium regulation in rat exocrine pancreas cells: an X-ray microanalytical study

Journal Article · Tue Mar 01 04:00:00 EST 1988 · Scan. Microsc.; (United States)

OSTI ID:6994991

Roos, N

We analysed four subcellular compartments in rat exocrine pancreas cells, zymogen granules, cytoplasm surrounding the zymogen granules, mitochondria and cytoplasm in the basal part of the cells for sodium, magnesium, phosphorus, sulfur, chlorine, potassium and calcium content, using ultrathin frozen-dried cryosections. The highest concentrations of calcium were measured in the zymogen granules and the surrounding apical part of the cell containing Golgi apparatus, smooth endoplasmic reticulum and condensing vacuoles. Calcium concentrations in the basal part of the cells (mostly rough endoplasmic reticulum) were 60% lower than in the apical part of the cells. The lowest calcium concentrations were measured in mitochondria. The results suggest that other subcellular compartments than the rough endoplasmic reticulum and mitochondria might be involved in the intracellular Ca2+ regulation.

• Research Organization: Univ. of Oslo (Norway)
• OSTI ID: 6994991
• Journal Information: Scan. Microsc.; (United States), Journal Name: Scan. Microsc.; (United States) Vol. 2:1; ISSN SCMIE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BIOSYNTHESIS
BODY
CALCIUM
CELL CONSTITUENTS
CHEMICAL ANALYSIS
CYTOPLASM
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ELECTRON MICROPROBE ANALYSIS
ELEMENTS
ENDOCRINE GLANDS
ENDOPLASMIC RETICULUM
GLANDS
HOMEOSTASIS
IONIZING RADIATIONS
MAMMALS
METALS
MICROANALYSIS
MITOCHONDRIA
ORGANOIDS
ORGANS
PANCREAS
RADIATIONS
RATS
RODENTS
SAMPLE PREPARATION
SYNTHESIS
VERTEBRATES
X RADIATION