Scavenger receptor-mediated endocytosis by sinusoidal cells in rat bone marrow

Thesis/Dissertation · Wed Dec 31 23:00:00 EST 1986

OSTI ID:6994848

Geoffroy, J S

Endocytosis of serum albumin by sinusoidal endothelial cells in rat bone marrow was investigated initially at the ultrastructural level with subsequent biochemical investigation of the specificity mediating this event. Bovine serum albumin adsorbed to 20nm colloidal gold particles (AuBSA) was chosen as the electron microscopic probe. Morphological data strongly suggested that a receptor was involved in uptake of AuBSA. Confirmation of receptor involvement in the uptake of AuBSA by marrow sinusoidal endothelial cells was achieved utilizing an in situ isolated hind limb perfusion protocol in conjunction with unlabeled, radiolabeled, and radio-/colloidal gold labeled probes. The major findings of competition and saturation experiments were: (1) endocytosis of AuBSA was mediated by a receptor for modified/treated serum albumin; (2) endocytosis of formaldehyde-treated serum albumin was mediated by a binding site which may be the same or closely related to the site responsible for the uptake of AuBSA; and (3) endocytosis of native untreated albumin was not mediated by receptor and probably represents fluid-phase pinocitosis.

• Research Organization: Illinois Univ., Chicago (USA)
• OSTI ID: 6994848
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
BONE MARROW
CATTLE
CHEMICAL ANALYSIS
COLLOIDS
DISPERSIONS
DOMESTIC ANIMALS
ELECTRON MICROPROBE ANALYSIS
ENDOTHELIUM
GOLD ISOTOPES
HEMATOPOIETIC SYSTEM
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MICROANALYSIS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTOSIS
PROTEINS
RATS
RECEPTORS
RODENTS
RUMINANTS
TISSUES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES