Interferon. beta. /sub 2//B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells
One of the oldest and most preserved of the homeostatic responses of the body to injury is the acute phase protein response associated with inflammation. The liver responds to hormone-like mediators by the increased synthesis of a series of plasma proteins called acute phase reactants. In these studies, the authors examined the relationship of hepatocyte-stimulating factor derived from peripheral blood monocytes to interferon ..beta../sub 2/ (IFN-..beta../sub 2/), which has been cloned. Antibodies raised against fibroblast-derived IFN-..beta.. having neutralizing activity against both IFN-..beta../sub 1/ and ..beta../sub 2/ inhibited the major hepatocyte-stimulating activity derived from monocytes. Fibroblast-derived mediator elicited the identical stimulated response in human HepG2 cells and primary rat hepatocytes as the monocyte cytokine. Finally, recombinant-derived human B-cell stimulatory factor type 2 (IFN-..beta../sub 2/) from Escherichia coli induced the synthesis of all major acute phase proteins studied in human hepatoma HepG2 and primary rat hepatocyte cultures. These data demonstrate that monocyte-derived hepatocyte-stimulating factor and IFN-..beta../sub 2/ share immunological and functional identity and that IFN-..beta../sub 2/, also known as B-cell stimulatory factor and hybridoma plasmacytoma growth factor, has the hepatocyte as a major physiologic target and thereby is essential in controlling the hepatic acute phase response.
- Research Organization:
- McMaster Univ., Hamilton, ON (Canada)
- OSTI ID:
- 6994629
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:20
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INFLAMMATION
GENETICS
PROTEINS
GENE REGULATION
ANTIBODIES
FIBROBLASTS
IMMUNITY
INJURIES
INTERFERON
LIVER CELLS
LYMPHOCYTES
LYMPHOKINES
MAN
MONOCYTES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PRIMATES
SOMATIC CELLS
SYMPTOMS
VERTEBRATES
550400* - Genetics