Induction and regulation of mRNA encoding 26-kDa protein in human cell lines treated with recombinant human tumor necrosis factor

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.13.4557· OSTI ID:5409657

Defilippi, P; Poupart, P; Tavernier, J; Fiers, W; Content, J

A 26-kDa protein, originally described in human fibroblasts superinduced for interferon ..beta.. (IFN-..beta..) production, and termed IFN-..beta../sub 2/ by other investigators, is induced by cycloheximide and by a 22-kDa, interleukin 1 (IL-1)-related factor. Although the structure and sequence of the corresponding gene show nonhomology with the IFN-..beta.. gene, the gene is identical to that of B-cell stimulatory factor 2, a human interleukin, and displays a very potent growth and differentiation factor activity for B lymphocytes. In this work the authors show that IL-1..beta.. and tumor necrosis factor (TNF) strongly induce the 26-kDa protein in FS-4 fibroblasts and in some transformed cell lines. Addition of cycloheximide to recombinant (r)IL-1..beta.. and rTNF further enhances the level of 26-kDa-protein mRNA. They determined the kinetics of induction and the amounts of rTNF and rIL-1..beta.. required for optimal induction of this mRNA in FS-4 cells and in HeLa H21 cells and found that rIL-1..beta.. is more efficient inducer of 26-kDa protein mRNA than is TNF. By analyzing the inducibility of the 26-kDa protein gene by rTNF and rIL-1..beta.. in a series of transformed cell lines that differ in their sensitivity to the cytotoxic action of TNF, they report a direct correlation between the 26-kDa protein mRNA expression and the resistance of these cells to the cytotoxic effect of TNF.

• Research Organization: Institut Pasteur du Brabant, Bruxelles (Belgium)
• OSTI ID: 5409657
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:13; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
CYCLOHEXIMIDE
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
FIBROBLASTS
FUNGICIDES
GENETICS
GROWTH FACTORS
HELA CELLS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LYMPHOKINES
MAMMALS
MAN
MESSENGER-RNA
MITOGENS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PESTICIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
SOMATIC CELLS
VERTEBRATES