Contribution of several amino acids and lactate to gluconeogenesis in hepatocytes isolated from rats fed various diets
- Univ. of California, Davis (USA)
The contribution under various nutritional regimens of several amino acids and lactate to gluconeogenesis was estimated by measuring the glucose formation from 14C-labeled substrates. Isolated rat hepatocytes were incubated for 60 min in a Krebs-Ringer bicarbonate buffer pH 7.4 containing lactate, pyruvate and all the amino acids at concentrations similar to their physiological levels found in rat plasma, with one precursor labeled in each flask. In all conditions, lactate was the major glucose precursor, providing over 60% of the glucose formed. Glutamine and alanine were the major amino acid precursors of glucose, contributing 9.8% and 10.6% of the glucose formed, respectively, in hepatocytes isolated from starved rats. Serine, glycine and threonine also contributed to gluconeogenesis in the starved liver cells at 2.6, 2.1 and 3.8%, respectively, of the glucose formed. The rate of glucose formation from the isolated hepatocytes of the starved rats and those fed either high protein or high fat was higher than that from rats fed a nonpurified diet.
- OSTI ID:
- 6994495
- Journal Information:
- Journal of Nutrition; (USA), Journal Name: Journal of Nutrition; (USA) Vol. 120:1; ISSN 0022-3166; ISSN JONUA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
DIET
FASTING
GLUCOSE
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTATES
LIVER CELLS
MAMMALS
METABOLISM
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES