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Inhibition of glycogen synthesis in rat hepatocytes by medium Zn/sup 2 +/

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)

In hepatocytes from fasted rats, Zn/sup 2 +/ in the range from 0 to 500..mu..M has relatively minor effects on gluconeogenesis from most substrates, or on ureagenesis from NH/sub 3/. In hepatocytes from fed rats, Zn/sup 2 +/ does not affect glycogenolysis. In hepatocytes from fasted rats, in which glycogen is being actively synthesized using the substrate combination of glycose, lactate and glutamine (all 10mM), Zn/sup 2 +/ markedly inhibits glucogen synthesis, with total inhibition at 500..mu..M, and a half maximal effect in the range from 50 to 100..mu..M. Dipicolinate (pyridine 2,6-dicarboxylate), a zinc chelator, is about as effective as L-glutamine in activating glycogen synthesis with the substrate combination of dihydroxyacetone, lactate and glucose (all 10mM). This suggests the possible hypothesis that endogenous Zn/sup 2 +/ might control the rate of glycogen synthesis in vivo. However, alternate explanations such as metabolite accumulation are also possible, since dipicolinate causes inhibition of gluconeogenesis from L-lactate. 28 references, 3 tables.

Research Organization:
Cedars-Sinai Medical Center, Los Angeles, CA
OSTI ID:
5970100
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 122:2; ISSN BBRCA
Country of Publication:
United States
Language:
English

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Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AMIDES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
ELEMENTS
GLUCOSE
GLUTAMINE
GLYCOGEN
HEXOSES
INHIBITION
IONS
LACTATES
LIVER CELLS
MAMMALS
METALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
ZINC
ZINC COMPOUNDS