Radiolocalization of monoclonal antibodies in hepatic metastases from human colon cancer in congenitally athymic mice

Journal Article · Thu Feb 01 04:00:00 EST 1990 · Cancer Research; (USA)

OSTI ID:6993954

Yoshida, K; Rivoire, M; Divgi, C; Welt, S; Cohen, A M; Sigurdson, E R ^[1]

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1. Memorial Sloan-Kettering Cancer Center, New York, NY (USA)

Intrasplenic injection of the HT-29 LMM metastatic human colon cancer line reproducibly results in hepatic metastasis formation in congenitally athymic mice. HT-29-15, a murine monoclonal antibody (mAb) of the IgG1 class reactive with the HT-29 LMM line, and BL-3, an isotype-matched control antibody, were labeled with 125I. Labeled mAbs were injected i.v. in mice with hepatic metastases, and animals were sacrificed on days 3, 5, and 7. Specific mAb uptake by tumor was significantly greater than nonspecific mAb uptake, as evidenced by specific/nonspecific tumor/blood ratios (radiolocalization indices) of 3.47/1-25.6/1. Relative mAb uptake was greater by the hepatic tumors than by the splenic tumors from day 3 to day 7, although this was significant (P less than 0.05) only on day 7 (5.12 {plus minus} 2.97 versus 1.79 {plus minus} 0.71). Tumor/uninvolved tissue ratios were also significantly greater (P less than 0.05) for the hepatic metastases than for the splenic tumors on day 7 (12.23 {plus minus} 3.85 versus 6.63 {plus minus} 2.63). This murine hepatic metastasis model appears useful for evaluation of localization of mAbs to hepatic metastases from human colon carcinoma.

• OSTI ID: 6993954
• Journal Information: Cancer Research; (USA), Journal Name: Cancer Research; (USA) Vol. 50; ISSN CNREA; ISSN 0008-5472
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL NEOPLASMS
GASTROINTESTINAL TRACT
GLANDS
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLING
LARGE INTESTINE
LIVER
MAMMALS
METASTASES
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
SPLEEN
TISSUE DISTRIBUTION
UPTAKE
VERTEBRATES