Activation of the rat kidney mineralocorticoid receptor

Journal Article · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

DOI:https://doi.org/10.1210/endo-119-4-1419· OSTI ID:6992216

Eisen, L P; Harmon, J M

Activation of the rat kidney mineralocorticoid receptor was investigated using DEAE-cellulose, DNA-cellulose and gel permeation chromatography. Specific labeling of the mineralocorticoid receptor was achieved by labeling with (/sup 3/H)aldosterone in the presence of the pure glucocorticoid RU28362. The specificity of labeling was confirmed by the lack of immunoreactivity of (/sup 3/H)aldosterone-labeled material with the monoclonal antiglucocorticoid receptor antibody BUGR-1. The unactivated aldosterone-mineralocorticoid receptor complex did not bind to DNA-cellulose, was eluted from DEAE-cellulose at relatively high salt (190 mM KCl) concentration and had an apparent Stokes radius when chromatographed on Sephacryl S300 of 6.3 nm. After activation (at 25/sup 0/C for 20 min), the aldosterone-mineralocorticoid receptor complex had increased affinity for DNA-cellulose, decreased affinity for DEAE-cellulose and appeared as a smaller complex when chromatographed on Sephacryl S300. These changes were blocked by sodium molybdate. The authors results indicate that activation of the rat kidney mineralocorticoid receptor is analogous to activation of the glucocorticoid receptor and suggest that activation of the mineralocorticoid receptor involves dissociation of a multimeric receptor form.

• Research Organization: Uniformed Services Univ. of the Health Science, Bethesda, MD
• OSTI ID: 6992216
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AFFINITY
ALDEHYDES
ALDOSTERONE
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BODY
CHROMATOGRAPHY
CORTICOSTEROIDS
HORMONES
HYDROXY COMPOUNDS
IMMUNE REACTIONS
KETONES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MEMBRANE PROTEINS
MINERALOCORTICOIDS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
TRITIUM COMPOUNDS
VERTEBRATES