Activation of the rat kidney mineralocorticoid receptor

Journal Article · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

DOI:https://doi.org/10.1210/endo-119-4-1419· OSTI ID:6992216

Eisen, L P; Harmon, J M

Activation of the rat kidney mineralocorticoid receptor was investigated using DEAE-cellulose, DNA-cellulose and gel permeation chromatography. Specific labeling of the mineralocorticoid receptor was achieved by labeling with (/sup 3/H)aldosterone in the presence of the pure glucocorticoid RU28362. The specificity of labeling was confirmed by the lack of immunoreactivity of (/sup 3/H)aldosterone-labeled material with the monoclonal antiglucocorticoid receptor antibody BUGR-1. The unactivated aldosterone-mineralocorticoid receptor complex did not bind to DNA-cellulose, was eluted from DEAE-cellulose at relatively high salt (190 mM KCl) concentration and had an apparent Stokes radius when chromatographed on Sephacryl S300 of 6.3 nm. After activation (at 25/sup 0/C for 20 min), the aldosterone-mineralocorticoid receptor complex had increased affinity for DNA-cellulose, decreased affinity for DEAE-cellulose and appeared as a smaller complex when chromatographed on Sephacryl S300. These changes were blocked by sodium molybdate. The authors results indicate that activation of the rat kidney mineralocorticoid receptor is analogous to activation of the glucocorticoid receptor and suggest that activation of the mineralocorticoid receptor involves dissociation of a multimeric receptor form.

• Research Organization: Uniformed Services Univ. of the Health Science, Bethesda, MD
• OSTI ID: 6992216
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MINERALOCORTICOIDS
RECEPTORS
BIOCHEMICAL REACTION KINETICS
LABELLING
AFFINITY
ALDOSTERONE
CHROMATOGRAPHY
IMMUNE REACTIONS
KIDNEYS
MONOCLONAL ANTIBODIES
RATS
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALDEHYDES
ANIMALS
ANTIBODIES
BODY
CORTICOSTEROIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques