Alkylation damage repair in mammalian genomes

Conference · Wed Jan 01 04:00:00 EST 1992

OSTI ID:6991958

Mitra, S; Roy, R; Kim, N K ^[1]; Tano, K ^[2]; Ibeanu, G C ^[3]; Dunn, W C

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1. Texas Univ. Medical Branch, Galveston, TX (United States). Sealy Center for Molecular Science Oak Ridge National Lab., TN (United States)
2. Banyu Pharmaceutical Co. Research Inst., Tsukuba (Japan) Oak Ridge National Lab., TN (United States)
3. NIEHS, Research Triangle, NC (United States) Oak Ridge National Lab., TN (United States)

The repair of O{sup 6} -alkylguanine in DNA involves only O{sup 6} -methyltransferase (MGMT) while the repair of N-alkylpurines requires multiple proteins including N-methylpurine-DNA glycosylase (MPG). While the biochemical properties human and mouse MGMTs are very similar, the mouse MPG removes 7-methylguanine more efficiently than the human protein. An increased level of MGMT, without a change in the level of MPG associated with gene amplification, was observed in a mouse cell line resistant to 2-chloroethyl-N-nitrosourea. In contrast, no correlation was observed between MPG level and resistance to methyl methanesulfonate in Chinese hamster ovary (CHO) cells. This result suggests a protein other than MPG limits the repair rate of N-alkylpurine in CHO cells.

• Research Organization: Oak Ridge National Lab., TN (United States)
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6991958
• Report Number(s): CONF-9208143-1; ON: DE93001208
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBON-GROUP TRANSFERASES
CLONING
DNA ADDUCTS
DNA HYBRIDIZATION
DNA REPAIR
DNA-CLONING
ENZYME INDUCTION
ENZYMES
GENE AMPLIFICATION
GENE REGULATION
HYBRIDIZATION
KINETICS
MAMMALS
MAN
METHYL TRANSFERASES
MICE
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
REACTION KINETICS
REPAIR
RODENTS
TRANSFERASES
VERTEBRATES