Alkylation damage repair in mammalian genomes
Conference
·
OSTI ID:10180082
- Texas Univ. Medical Branch, Galveston, TX (United States). Sealy Center for Molecular Science
- Banyu Pharmaceutical Co. Research Inst., Tsukuba (Japan)
- NIEHS, Research Triangle, NC (United States)
- Oak Ridge National Lab., TN (United States)
- Leiden Univ. (Netherlands). Sylvius Labs.
- Kernforschungszentrum Karlsruhe GmbH (Germany). Inst. fuer Genetik und Toxikologie
The repair of O{sup 6} -alkylguanine in DNA involves only O{sup 6} -methyltransferase (MGMT) while the repair of N-alkylpurines requires multiple proteins including N-methylpurine-DNA glycosylase (MPG). While the biochemical properties human and mouse MGMTs are very similar, the mouse MPG removes 7-methylguanine more efficiently than the human protein. An increased level of MGMT, without a change in the level of MPG associated with gene amplification, was observed in a mouse cell line resistant to 2-chloroethyl-N-nitrosourea. In contrast, no correlation was observed between MPG level and resistance to methyl methanesulfonate in Chinese hamster ovary (CHO) cells. This result suggests a protein other than MPG limits the repair rate of N-alkylpurine in CHO cells.
- Research Organization:
- Oak Ridge National Lab., TN (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 10180082
- Report Number(s):
- CONF-9208143--1; ON: DE93001208
- Country of Publication:
- United States
- Language:
- English
Similar Records
Alkylation damage repair in mammalian genomes
Characterization of cDNA encoding mouse DNA repair protein O sup 6 -methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli
Overexpressed human metallothionein IIA gene protects Chinese hamster ovary cells from killing by alkylating agents
Conference
·
Tue Dec 31 23:00:00 EST 1991
·
OSTI ID:6991958
Characterization of cDNA encoding mouse DNA repair protein O sup 6 -methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli
Journal Article
·
Mon Feb 24 23:00:00 EST 1992
· Biochemistry; (United States)
·
OSTI ID:5562711
Overexpressed human metallothionein IIA gene protects Chinese hamster ovary cells from killing by alkylating agents
Journal Article
·
Sat Mar 31 23:00:00 EST 1990
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:6904776
Related Subjects
550200
560300
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
CHEMICALS METABOLISM AND TOXICOLOGY
DNA ADDUCTS
DNA REPAIR
DNA-CLONING
ENZYME INDUCTION
GENE AMPLIFICATION
MAN
METHYL TRANSFERASES
MICE
560300
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
CHEMICALS METABOLISM AND TOXICOLOGY
DNA ADDUCTS
DNA REPAIR
DNA-CLONING
ENZYME INDUCTION
GENE AMPLIFICATION
MAN
METHYL TRANSFERASES
MICE