Use of fast atom bombardment mass spectrometry (FAB MS) for the analysis of zinc stable isotopes in biological samples

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6991125

Peirce, P; Fennessey, P; Hambidge, M; Miller, L; Goss, C

The use of stable isotopes offer a means of measuring human metabolism of trace metals in populations in which radioisotopes are contraindicated. The objective of this research has been to develop stable isotope methodology for the measurement of zinc in biological samples and to apply this methodology in pilot studies of intestinal zinc absorption. Analyses were made with a VG 7070E HF sector mass spectrometer at a resolution of 3000. Samples of 10 ..mu..l containing approximately 1 ..mu..g Zn were applied directly to a gold target, dried and inserted into the FAB source for ionization. Precision at the 1% level of enrichment was 2% (RSD). Pilot studies of the clinical application have been undertaken on two subjects to each of whom a single oral dose of enriched /sup 70/Zn was administered after an overnight fast. A comparison of aqueous standards and prepared fecal samples showed undetectable levels of contamination at the /sup 64/Zn to /sup 70/Zn masses. Precision of fecal samples is comparable to that of aqueous standards. Calculated absorption after an overnight fast was between 60-65% of the administered dose. The FAB MS provides a simple, sensitive and precise technique for the measurement of zinc stable isotopes in biological samples.

• Research Organization: Univ. of Colorado Health Sciences Center, Denver
• OSTI ID: 6991125
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ZINC
INTESTINAL ABSORPTION
METABOLISM
FECES
MASS SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
ZINC 64
ZINC 70
ABSORPTION
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
ELEMENTS
EVEN-EVEN NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
METALS
NUCLEI
SPECTROSCOPY
UPTAKE
WASTES
ZINC ISOTOPES
550501* - Metabolism- Tracer Techniques