Analysis of stable isotopes in urine to determine the fractional absorption (FA) of zinc
Conference
·
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
OSTI ID:5146503
- Univ. of Colorado, Denver (United States) Memorial Univ., St. John's, Newfoundland (Canada)
{sup 68}Zn and {sup 70}Zn were administered orally or intravenously to 4 normal adults to determine fractional absorption (FA) of zinc. Urine and fecal samples were collected for 7 days after giving the isotopes. Feces were dried, ashed and passed through AG-1 resin columns to separate zinc from other trace elements. Urine was evaporated, ashed and passed through Chelex-100 resin to separate Ca, Mg, P, and K before passing eluent through AG-1 resin. All samples were analyzed by Fast Atom Bombardment Mass Spectrometry. From 32 hours onwards the enrichment of {sup 68}Zn and {sup 70}Zn in urine declined proportionately so that FA could be determined according to the formula: FA - Enrichment (Oral/IV) {times} Dose (IV/Oral). FA was also determined from total cumulative fecal excretion of the oral isotopes. FA determinations from urine/feces for each of the four subjects (ZK{sup 1}-ZK{sup 4}) were: ZK1-.79 {plus minus} 03/.70 {plus minus} .01; ZK2 .79 {plus minus} .05/.69 {plus minus} .02; ZK3-.25 {plus minus} .01/.25 {plus minus} .01; and ZK4-.39 {plus minus} .02/.37 {plus minus}.02. Subjects ZK1 and ZK2 received the oral isotope without food while ZK3 and ZK4 received their oral isotope with meals. The authors conclude that FA of zinc can be determined by measurement of isotope enrichment in urine.
- OSTI ID:
- 5146503
- Report Number(s):
- CONF-9104107--
- Conference Information:
- Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Measurement of an exchangeable pool of zinc by analysis of stable isotope tracer in plasma and urine
Appearance of infused zinc ( sup 70 Zn) and oral zinc ( sup 68 Zn) in breast milk
Intrinsic and extrinsic zinc absorption by infants from cow milk formula
Conference
·
Thu Mar 14 23:00:00 EST 1991
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
·
OSTI ID:5215065
Appearance of infused zinc ( sup 70 Zn) and oral zinc ( sup 68 Zn) in breast milk
Conference
·
Thu Mar 14 23:00:00 EST 1991
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
·
OSTI ID:5146520
Intrinsic and extrinsic zinc absorption by infants from cow milk formula
Conference
·
Fri Feb 28 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5461758
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
ELEMENTS
EVEN-EVEN NUCLEI
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
ISOTOPES
MASS SPECTROSCOPY
MATERIALS
METABOLISM
METALS
NUCLEI
ORAL ADMINISTRATION
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
URINE
WASTES
ZINC
ZINC 68
ZINC 70
ZINC ISOTOPES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
ELEMENTS
EVEN-EVEN NUCLEI
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
ISOTOPES
MASS SPECTROSCOPY
MATERIALS
METABOLISM
METALS
NUCLEI
ORAL ADMINISTRATION
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
URINE
WASTES
ZINC
ZINC 68
ZINC 70
ZINC ISOTOPES