Increased lysophosphatidylinositol levels in raw 264. 7 macrophage tumor cells during stimulation by a monosaccharide precursor of Escherichia coli lipid
N/sup 2/,O/sup 3/-diacylglucosamine-1-phosphate (lipid X), a biosynthetic precursor of E. coli lipid A, has been isolated from a mutant strain of E. coli, and has also been chemically synthesized. These compounds were used to stimulate macrophage tumor cells in culture to examine their effects on animal cell lipid metabolism. The addition of either substance (2-5 ..mu..M) to cells that had been labeled for several generations with /sup 32/P/sub i/ resulted in a 4-6 fold increase in the labeling of lysophosphatidylinositol (LPI). A decrease in the labeling of phosphatidylinositol was the only other significant change in the phospholipid profile. LPI labeling reached a maximum at 45 minutes after the addition of lipid X, followed by a decline to near control values within 2 hours. This effect was dependent upon extracellular calcium and was inhibited by 95% when cycloheximide (5 ..mu..g/ml) was added at the time of stimulation. The addition of lipopolysaccharide (1 ..mu..g/ml), phorbol 12-myristate 13-acetate (2 x 10/sup -7/ M) and calcium ionophore (10/sup -6/ M) also resulted in an increase in LPI labeling. The effect of calcium ionophore on LPI labeling was not inhibited by cycloheximide. The ability of an agent to elicit this response did not correlate with its ability to induce the release of prostaglandins.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 6984938
- Report Number(s):
- CONF-8606151-; TRN: 86-039298
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
LIPIDS
METABOLISM
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PHOSPHATES
PHOSPHOLIPIDS
LABELLING
CALCIUM COMPOUNDS
ESCHERICHIA COLI
LIPOPOLYSACCHARIDES
MACROPHAGES
PHOSPHORUS 32
PRECURSOR
TRACER TECHNIQUES
TUMOR CELLS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARCINOGENS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ESTERS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHAGOCYTES
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques