/sup 3/H arachidonic acid incorporation and metabolism in purified human basophils

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5325475

Warner, J A; Peters, S P; Lichtenstein, L M; MacGlashan, Jr, D W

A central feature of the allergic response is the generation of arachidonic acid (AA) metabolites by basophils and mast cells. In addition, AA metabolism may play a role in regulating the anti-IgE mediated degranulation of human basophils. To study this biochemistry, purified human basophils (>80%) were labeled with /sup 3/H-AA (0.3 ..mu..M, 25 ..mu..Ci/ml, 2 hours at 37/sup 0/C) and subsequently challenged with anti-IgE. Basophils were found to incorporate 45 +/- 3% of the exogenous AA which distributed into phospholipids (PL) (77.1 +/- 3.5%) and neutral lipids (19.7 +/- 3.3%) with only 5.3 +/- 2.7% remaining as the free acid (n = 7). Phosphatidylcholine (23.9 +/- 1.7%), phosphatidylinositol (22.0 +/- 1.4%) and phosphatidylethanolamine (14.5 +/- 2.7%) accounted for the majority of the AA with the remaining PL containing <3%. Anti-IgE (0.1 ..mu..g/ml) challenge led to the release of histamine (23.8 +/- 4.7%) and /sup 3/H-AA (8.1 +/- 1.7%) (n = 5). HPLC analysis revealed unmetabolized /sup 3/H-AA, /sup 3/H-LTC/sub 4/, /sup 3/H-HETE and an unidentified peak which migrated in the prostaglandin region of the elution profile. The same metabolites were released when the basophils were challenged with antigen. The calcium ionophore A23187 (1..mu..g/ml) also caused the release of histamine (37.4 +/- 4.1%) and /sup 3/H-AA (17.0 +/- 2.9%), while the phorbol ester, TPA caused HR (19.7 +/- 5.8%) but no increase in /sup 3/H AA turnover. Because of limited cell numbers this is the first time the authors have been able to study AA metabolism in human basophils.

• Research Organization: Johns Hopkins Univ. School of Medicine, Baltimore, MD
• OSTI ID: 5325475
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ALLERGY
AMINES
ANIMAL CELLS
ANIMALS
ANTIGENS
ARACHIDONIC ACID
AZOLES
BASOPHILS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM
CARBOXYLIC ACIDS
CARCINOGENS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
ELEMENTS
ESTERS
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MAST CELLS
MATERIALS
METABOLISM
METABOLITES
METALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PATHOLOGICAL CHANGES
PHORBOL ESTERS
PHOSPHOLIPIDS
PRIMATES
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES