Differential release of mediators from human basophils: Differences in arachidonic acid metabolism following activation by unrelated stimuli

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · J. Leukocyte Biol.; (United States)

OSTI ID:5722446

Warner, J A; Peters, S P; Lichtenstein, L M; Hubbard, W; Yancey, K B; Stevenson, H C; Miller, P J; MacGlashan, Jr, D W

We have examined the release of histamine and LTC4 from purified human basophils challenged with several different stimuli, both physiological and nonphysiological. Basophils (n = 16) challenged with 0.1 micrograms/ml anti-IgE released 38 +/- 4% of their available histamine and 39 +/- 12 ng LTC4/10(6) basophils within 15-30 min. F-Met peptide (n = 8) caused the release of 54 +/- 8% histamine and 42 +/- 25 ng LTC4/10(6) basophils within a period of 2-5 min. C5a caused the release of 22 +/- 3% histamine from selected donors but failed to initiate any LTC4 release unless combined with D2O or 5 mM extracellular calcium. The two nonphysiological stimuli A23187 and TPA caused extensive histamine release, 67 +/- 8 and 82 +/- 11%, respectively, and while A23187 initiated a large and rapid release of leukotriene, TPA failed to release any LTC4 even when combined with D2O or 2-5 mM extracellular calcium. Increased concentrations of extracellular calcium enhanced anti-IgE and f-Met peptide induced release of LTC4 but inhibited the A23187 induced release of leukotriene. A single peak of immunoreactive leukotriene C4 that comigrated with the authentic standard was identified using HPLC followed by radioimmunoassay. No LTD4 or LTE4 could be detected. Purified human basophils incubated with 0.2 microM (3H)AA incorporated 290 pmol/10(6) cells, or 32 +/- 5% of the available label within 60 min. The (3H)AA was taken principally into the phospholipids (73 +/- 5%), with 20 +/- 3% as neutral lipid, and only 5 +/- 2% remaining as the free acid. Three phospholipid subclasses, phosphatidylcholine, PC (24 +/- 2%), phosphatidylinositol, PI (22 +/- 1%), and phosphatidylethanolamine, PE (15 +/- 3%), accounted for the majority of the incorporated (3H)AA while the remainder of the phospholipids accounted for less than 5% of the total cpm.

• Research Organization: Johns Hopkins Univ. School of Medicine, Baltimore, MD (USA)
• OSTI ID: 5722446
• Journal Information: J. Leukocyte Biol.; (United States), Journal Name: J. Leukocyte Biol.; (United States) Vol. 45:6; ISSN JLBIE
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
AMINES
AMINO ACIDS
ANIMALS
ARACHIDONIC ACID
AZOLES
BASOPHILS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM
CARBOXYLIC ACIDS
CARCINOGENS
CHROMATOGRAPHY
COMPLEMENT
DEUTERIUM
ELEMENTS
ESTERS
GLOBULINS
HETEROCYCLIC COMPOUNDS
HISTAMINE
HYDROGEN ISOTOPES
IMIDAZOLES
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
METABOLISM
METALS
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDES
PHENYLALANINE
PHORBOL ESTERS
PHOSPHOLIPIDS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SECRETION
SEPARATION PROCESSES
STABLE ISOTOPES
STIMULI
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES