Cadmium inhibition of L-alanine transport into renal brush border membrane vesicles isolated from the winter flounder (Pseudopleuronectes americanus)

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Toxicology and Applied Pharmacology; (USA)

DOI:https://doi.org/10.1016/0041-008X(89)90194-4· OSTI ID:6983235

Bevan, C; Kinne-Saffran, E; Foulkes, E C; Kinne, R K ^[1]

---

1. Max-Planck-Institut fuer Systemphysiologie, Dortmund (Germany, F.R.)

Using isolated brush border membrane vesicles from the kidney of the winter flounder (Pseudopleuronectes americanus), we have studied the effect of cadmium on L-alanine transport. Pretreatment of vesicles with 0.1 mM Cd{sup 2+} resulted in inhibition of L-alanine uptake in the presence of a NaCl (but not KCl) gradient. Inhibition was due to a specific interaction with the sodium-alanine cotransport system and not a change in the driving forces for alanine transport, since Cd{sup 2+} did not affect sodium-dependent D-glucose uptake. The effect of Cd{sup 2+} on Na{sup +}-alanine cotransport showed mixed-type inhibition which is only partially reversible by EDTA. Cd{sup 2+} uptake itself was shown to be time and temperature dependent, resulting in binding to both sides of the membrane. No direct correlation was possible between inhibition of L-alanine transport and the amount of Cd{sup 2+} taken up by the membranes. Nevertheless, the striking time dependence of the effect of Cd{sup 2+} on sodium-dependent L-alanine uptake and the inability of EDTA to reverse the inhibitory action of Cd{sup 2+} suggest that Cd{sup 2+} inhibits Na+-alanine cotransport at the cytoplasmic side of the membrane.

• OSTI ID: 6983235
• Journal Information: Toxicology and Applied Pharmacology; (USA), Journal Name: Toxicology and Applied Pharmacology; (USA) Vol. 101:3; ISSN TXAPA; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALANINES
ALKALI METALS
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
BODY
CADMIUM
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CYTOPLASM
ELEMENTS
FISHES
INHIBITION
KIDNEYS
MEMBRANE TRANSPORT
MEMBRANES
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POLLUTION
PROTEINS
SODIUM
TIME DEPENDENCE
TOXICITY
VERTEBRATES
WATER POLLUTION