Peritubular uptake and brush border transport of SYMg by flounder renal tubules

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Am. J. Physiol.; (United States)

OSTI ID:6256003

Renfro, J L; Shustock, E

The uptake of SYMg by isolated renal tubules of winter flounder, Pseudopleuronectes americanus, was studied by compartmental analysis. Two phases of uptake were seen in steady-state conditions. The slow-exchanging compartment was 46% of the total Mg content, and uptake into this compartment was saturable and inhibited by 10 mM CaCl2, dinitrophenol, and ouabain but not by furosemide, Na-free medium, or cytochalasin B. The fast-exchanging compartment was 5% of the total Mg, and uptake into this compartment showed sigmoid saturation kinetics. The fast uptake rate was inhibited by dinitrophenol, ouabain, Na-free medium, 10 mM CaCl2, and furosemide but stimulated by cytochalasin B. SYMg efflux from brush border membrane vesicles (BBMV) was stimulated by both an inside positive electrical potential generated by 100 mM KCl and by 100 mM NaCl. BBMV uptake was inhibited by 10 mM CaCl2 and unaffected by furosemide. The relationship of electrical gradient-driven and Na gradient-driven Mg transport to the Mg secretory function of these tubules is discussed.

• Research Organization: Univ. of Connecticut, Storrs
• OSTI ID: 6256003
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM CHLORIDES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHLORIDES
CHLORINE COMPOUNDS
COMPARTMENTS
DINITROPHENOL
DRUGS
ELEMENTS
EVEN-EVEN NUCLEI
FISHES
GLYCOSIDES
HALIDES
HALOGEN COMPOUNDS
HOURS LIVING RADIOISOTOPES
HYDROXY COMPOUNDS
ISOTOPES
KIDNEYS
KINETICS
LIGHT NUCLEI
MAGNESIUM 28
MAGNESIUM ISOTOPES
MEMBRANE TRANSPORT
METALS
NITRO COMPOUNDS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OUABAIN
PHENOLS
PHYSIOLOGY
RADIOISOTOPES
SODIUM
STROPHANTHINS
TUBULES
UPTAKE
VERTEBRATES