Alteration of spectral characteristics of human artery wall caused by 476-nm laser irradiation

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Lasers in Surgery and Medicine; (USA)

DOI:https://doi.org/10.1002/lsm.1900090606· OSTI ID:6982776

Chaudhry, H W; Richards-Kortum, R; Kolubayev, T; Kittrell, C; Partovi, F; Kramer, J R; Feld, M S ^[1]

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1. Massachusetts Institute of Technology, Cambridge (USA)

Fluorescence spectroscopy is a promising new technique for discrimination of normal and atherosclerotic arterial tissues. It has been suggested that this technique be used as a guidance system for laser angiosurgery catheters; however, irradiation by 476-nm light can change the spectroscopic properties of arterial tissue. We present studies that establish intensity levels and exposure times at which alterations in tissue spectral properties are minimal. We also investigate the nature of spectral alterations following exposure of normal human aorta to high intensities of 476-nm laser light. Changes in laser-induced fluorescence (LIF) are characterized by two prominent features: the peak fluorescence intensity decreases permanently, and the fluorescence lineshape changes in a largely reversible way. We relate these changes to alterations in individual tissue chromophores: permanent changes in absolute fluorescence intensity are due to irreversible changes in tissue fluorophores, reversible changes in fluorescence lineshape are due alterations in tissue absorbers. A simple kinetic model is used to describe the decrease in absolute fluorescence intensity.

• OSTI ID: 6982776
• Journal Information: Lasers in Surgery and Medicine; (USA), Journal Name: Lasers in Surgery and Medicine; (USA) Vol. 9:6; ISSN 0196-8092; ISSN LSMED
• Country of Publication: United States
• Language: English

Related Subjects

550600 -- Medicine
560400* -- Other Environmental Pollutant Effects
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AORTA
ARTERIES
ARTERIOSCLEROSIS
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
COLLAGEN
DISEASES
ELECTROMAGNETIC RADIATION
FLUORESCENCE
LASER RADIATION
LUMINESCENCE
MAMMALS
MAN
MEDICINE
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIATIONS
SCLEROPROTEINS
SURGERY
VASCULAR DISEASES
VERTEBRATES