Five subunit genes of the human muscle nicotinic acetylcholine receptor are mapped to two linkage groups on chromosome 2 and 17

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1384· OSTI ID:6975660

Lobos, E A ^[1]

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1. Washington Univ., St. Louis, MO (United States)

RFLPs were detected in the five subunit genes of the human muscle nicotinic acetylcholine receptor (nAChR) using genomic DNA or cDNA probes from the homologous mouse loci. The RFLPs at the alpha-, beta-, gamma-, delta-, and epsilon-subunit gene loci were analyzed for genetic linkage in 16 families (n = 188). Significant evidence was obtained for close linkage of the [beta]- and [epsilon]-nAChR genes and much greater genetic distance between the [alpha]-nAChR gene and the [gamma]/[delta]-nAChR gene complex. The linkage analysis program CRI-MAP was used to map the positions of the [beta]- and [epsilon]-nAChR genes relative to seven markers on chromosome 17. The results indicate the [beta]- and [epsilon]-nAChR probes to a panel of human-hamster somatic cell hybrids. The [alpha]-, [gamma]-, and [delta]-nAChR genes were placed on a map of 13 chromosome 2 markers. The linkage analysis placed the nAChR genes at two sites on chromosome 2q about equidistant from the marker CRYGP1, with the [alpha]-nAChR gene about 27 cM proximal and the [gamma]/[delta]-nAChR gene complex about 31 cM distal to CRYGP1. 46 refs., 2 figs., 5 tabs.

• OSTI ID: 6975660
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:3; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
CHROMOSOMES
DRUGS
ESTERS
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 17
HUMAN CHROMOSOME 2
HUMAN CHROMOSOMES
MAPPING
MEMBRANE PROTEINS
NEUROREGULATORS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PROTEINS
QUATERNARY COMPOUNDS
RECEPTORS