The effect of cytidine-diphosphate choline (CDP-choline) on brain lipid changes during aging
Lipid synthesis has been tested in vivo in different brain areas of 12-month-old male rats. Cortex, striatum, brainstem, and subcortex of brain have been examined. The cerebellum was discarded. Mixtures of (2-/sup 3/H)glycerol and (Me-/sup 14/C)choline were injected into the lateral ventricle of the brain as lipid precursors, and their incorporation into total lipid, water-soluble intermediates and choline-containing phospholipids was examined 1 hr after isotope injection. In another series of experiments cytidine-5'-diphosphate choline (CDP-choline) was injected intraventricularly to the aged rats 10 min before sacrifice with a simultaneous injection, and radioactivity assays were performed as above. Distribution of radioactivity content of CDP-choline among brain areas 10 min after its administration showed a noticeable enrichment of the nucleotide and water-soluble-related compounds in the examined areas, but to a lesser degree in the cerebral cortex. The incorporation of labelled glycerol, which is severely depressed in aged rats in all four areas (Gaiti et al, 1982, 1983), was increased only in the cortex, and apparently decreased in the other areas. This last result is probably due to a dilution effect brought about by the administered cold CDP-choline upon the (/sup 14/C)-containing water-soluble metabolites. As a consequence, the (/sup 3/H)/(/sup 14/C) ratio in total lipid and in isolated phosphatidylcholine and choline plasmalogen increased after CDP-choline treatment.
- Research Organization:
- Department of Biochemistry, University of Perugia Medical School, Italy
- OSTI ID:
- 6975594
- Journal Information:
- J. Neurosci. Res.; (United States), Vol. 11:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHOLINE
METABOLISM
GLYCEROL
PHOSPHOLIPIDS
BIOSYNTHESIS
AGE DEPENDENCE
BRAIN
CARBON 14 COMPOUNDS
LIPIDS
TRITIUM COMPOUNDS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
BODY
CENTRAL NERVOUS SYSTEM
DRUGS
ESTERS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
QUATERNARY COMPOUNDS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques