Capillary liquid chromatography using laser-based and mass spectrometric detection: Technical progress report for the period September 1, 1987-August 31, 1988

Technical Report · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6975505

Sepaniak, M J; Cook, K D

The focus of this work is the development of capillary liquid chromatography, in particular an electrokinetic separation technique that we have termed micellar electrokinetic capillary chromatography (MECC), as a practical separation technique for the analyses of volume-limited complex samples and for difficult-to-resolve compounds. Most of our research during this period has involved fundamental and practical studies of the MECC technique. Solutes are separated in MECC in open capillary columns based upon their differential distribution between an electroosmotically-pumped mobile phase and an electrophoretically-retarded micellar phase. In our work the electrophoretic flow of the micelles opposes and is of a smaller magnitude than the electroosmotic flow. Consequently, solutes that partition between the mobile and micellar phases are eluted within a limited retention range. The main virtues of the MECC technique are exceptional efficiency and the fact that it can be used to separate samples containing charged, neutral, or mixtures of charged and neutral solutes. The restriction of solutes demonstrating at least moderate water solubility and the limited elution range of the technique are limitations of MECC. 13 refs.

• Research Organization: Tennessee Univ., Knoxville (USA). Dept. of Chemistry
• DOE Contract Number: FG05-86ER13613
• OSTI ID: 6975505
• Report Number(s): DOE/ER/13613-12; ON: DE88011183
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CHROMATOGRAPHY
EFFICIENCY
OPTIMIZATION
SPECIFICITY
ABSORPTION
ACETONITRILE
AFLATOXIN
AMINO ACIDS
CAPILLARIES
FLUORESCENCE
LASERS
MASS SPECTROSCOPY
METHANOL
MICELLAR SYSTEMS
ORGANIC SOLVENTS
PHENOLS
PROGRESS REPORT
SOLUBILITY
SOLVENT PROPERTIES
VITAMIN B GROUP
ALCOHOLS
ANTIGENS
AROMATICS
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DOCUMENT TYPES
HYDROXY COMPOUNDS
LUMINESCENCE
MATERIALS
NITRILES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
SEPARATION PROCESSES
SOLVENTS
SPECTROSCOPY
TOXIC MATERIALS
TOXINS
VITAMINS
400105* - Separation Procedures