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Title: Capillary liquid chromatography using laser-based and mass spectrometric detection: Technical progress report for the period September 1, 1987-August 31, 1988

Abstract

The focus of this work is the development of capillary liquid chromatography, in particular an electrokinetic separation technique that we have termed micellar electrokinetic capillary chromatography (MECC), as a practical separation technique for the analyses of volume-limited complex samples and for difficult-to-resolve compounds. Most of our research during this period has involved fundamental and practical studies of the MECC technique. Solutes are separated in MECC in open capillary columns based upon their differential distribution between an electroosmotically-pumped mobile phase and an electrophoretically-retarded micellar phase. In our work the electrophoretic flow of the micelles opposes and is of a smaller magnitude than the electroosmotic flow. Consequently, solutes that partition between the mobile and micellar phases are eluted within a limited retention range. The main virtues of the MECC technique are exceptional efficiency and the fact that it can be used to separate samples containing charged, neutral, or mixtures of charged and neutral solutes. The restriction of solutes demonstrating at least moderate water solubility and the limited elution range of the technique are limitations of MECC. 13 refs.

Authors:
;
Publication Date:
Research Org.:
Tennessee Univ., Knoxville (USA). Dept. of Chemistry
OSTI Identifier:
6975505
Report Number(s):
DOE/ER/13613-12
ON: DE88011183
DOE Contract Number:  
FG05-86ER13613
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; CHROMATOGRAPHY; EFFICIENCY; OPTIMIZATION; SPECIFICITY; ABSORPTION; ACETONITRILE; AFLATOXIN; AMINO ACIDS; CAPILLARIES; FLUORESCENCE; LASERS; MASS SPECTROSCOPY; METHANOL; MICELLAR SYSTEMS; ORGANIC SOLVENTS; PHENOLS; PROGRESS REPORT; SOLUBILITY; SOLVENT PROPERTIES; VITAMIN B GROUP; ALCOHOLS; ANTIGENS; AROMATICS; BLOOD VESSELS; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; DOCUMENT TYPES; HYDROXY COMPOUNDS; LUMINESCENCE; MATERIALS; NITRILES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; SEPARATION PROCESSES; SOLVENTS; SPECTROSCOPY; TOXIC MATERIALS; TOXINS; VITAMINS; 400105* - Separation Procedures

Citation Formats

Sepaniak, M J, and Cook, K D. Capillary liquid chromatography using laser-based and mass spectrometric detection: Technical progress report for the period September 1, 1987-August 31, 1988. United States: N. p., 1988. Web.
Sepaniak, M J, & Cook, K D. Capillary liquid chromatography using laser-based and mass spectrometric detection: Technical progress report for the period September 1, 1987-August 31, 1988. United States.
Sepaniak, M J, and Cook, K D. Fri . "Capillary liquid chromatography using laser-based and mass spectrometric detection: Technical progress report for the period September 1, 1987-August 31, 1988". United States.
@article{osti_6975505,
title = {Capillary liquid chromatography using laser-based and mass spectrometric detection: Technical progress report for the period September 1, 1987-August 31, 1988},
author = {Sepaniak, M J and Cook, K D},
abstractNote = {The focus of this work is the development of capillary liquid chromatography, in particular an electrokinetic separation technique that we have termed micellar electrokinetic capillary chromatography (MECC), as a practical separation technique for the analyses of volume-limited complex samples and for difficult-to-resolve compounds. Most of our research during this period has involved fundamental and practical studies of the MECC technique. Solutes are separated in MECC in open capillary columns based upon their differential distribution between an electroosmotically-pumped mobile phase and an electrophoretically-retarded micellar phase. In our work the electrophoretic flow of the micelles opposes and is of a smaller magnitude than the electroosmotic flow. Consequently, solutes that partition between the mobile and micellar phases are eluted within a limited retention range. The main virtues of the MECC technique are exceptional efficiency and the fact that it can be used to separate samples containing charged, neutral, or mixtures of charged and neutral solutes. The restriction of solutes demonstrating at least moderate water solubility and the limited elution range of the technique are limitations of MECC. 13 refs.},
doi = {},
url = {https://www.osti.gov/biblio/6975505}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {1}
}

Technical Report:
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